PI-3 Kinase p85 Subunit Alpha antibody | U5
Mouse anti PI-3 Kinase p85 Subunit Alpha
- Product Type
- Monoclonal Antibody
- Clone
- U5
- Isotype
- IgG1
- Specificity
- PI-3 Kinase p85 Subunit Alpha
Antibody Quality and Performance Guarantee
| Mouse anti PI-3 Kinase p85 Subunit alpha antibody, clone U5 recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Monkey Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Baculovirus expressed (Recombinant) Bovine p85 alpha subunit of PI 3 kinase.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunoprecipitation | |
||
| Western Blotting | |
1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for PI-3 Kinase p85 Subunit Alpha antibody
-
Reif, K. et al. (1993) Divergent regulation of phosphatidylinositol 3-kinase P85 alpha and P85 beta isoforms upon T cell activation.
J Biol Chem. 268 (15): 10780-8. -
Tatin, F. et al. (2010) Sodium fluoride induces podosome formation in endothelial cells.
Biol Cell. 102 (9): 489-98.
- RRID
- AB_1125343
- UniProt
- P23727
- P27986
- P26450
- Q63787
- Entrez Gene
- PIK3R1
- Pik3r1
- PIK3R1
- Pik3r1
- GO Terms
- GO:0043125 ErbB-3 class receptor binding
- GO:0005158 insulin receptor binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005943 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex
- GO:0008286 insulin receptor signaling pathway
- GO:0046326 positive regulation of glucose import
- GO:0035014 phosphatidylinositol 3-kinase regulator activity
- GO:0043560 insulin receptor substrate binding
- GO:0046854 phosphatidylinositol phosphorylation
- View More GO Terms
- GO:0048009 insulin-like growth factor receptor signaling pathway
- GO:0090004 positive regulation of establishment of protein localization in plasma membrane
- GO:0001953 negative regulation of cell-matrix adhesion
- GO:0006468 protein phosphorylation
- GO:0016303 1-phosphatidylinositol-3-kinase activity
- GO:0030183 B cell differentiation
- GO:0030335 positive regulation of cell migration
- GO:0043066 negative regulation of apoptosis
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0005545 1-phosphatidylinositol binding
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0019903 protein phosphatase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0043559 insulin binding
- GO:0044419 interspecies interaction between organisms
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0050900 leukocyte migration
- GO:0060396 growth hormone receptor signaling pathway
- GO:0005886 plasma membrane
- GO:0001878 response to yeast
- GO:0001934 positive regulation of protein phosphorylation
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0005516 calmodulin binding
- GO:0005625 soluble fraction
- GO:0005634 nucleus
- GO:0006006 glucose metabolic process
- GO:0030971 receptor tyrosine kinase binding
- GO:0007584 response to nutrient
- GO:0008022 protein C-terminus binding
- GO:0042493 response to drug
- GO:0009750 response to fructose stimulus
- GO:0010040 response to iron(II) ion
- GO:0010459 negative regulation of heart rate
- GO:0010628 positive regulation of gene expression
- GO:0046935 1-phosphatidylinositol-3-kinase regulator activity
- GO:0019717 synaptosome
- GO:0019901 protein kinase binding
- GO:0019904 protein domain specific binding
- GO:0022408 negative regulation of cell-cell adhesion
- GO:0030331 estrogen receptor binding
- GO:0031625 ubiquitin protein ligase binding
- GO:0032355 response to estradiol stimulus
- GO:0032570 response to progesterone stimulus
- GO:0033574 response to testosterone stimulus
- GO:0043200 response to amino acid stimulus
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0043551 regulation of phosphatidylinositol 3-kinase activity
- GO:0045471 response to ethanol
- GO:0045663 positive regulation of myoblast differentiation
- GO:0045768 positive regulation of anti-apoptosis
- GO:0045776 negative regulation of blood pressure
- GO:0045861 negative regulation of proteolysis
- GO:0048662 negative regulation of smooth muscle cell proliferation
- GO:0051117 ATPase binding
- GO:0051219 phosphoprotein binding
- GO:0051591 response to cAMP
- GO:0070848 response to growth factor stimulus
- GO:0071398 cellular response to fatty acid
- GO:0071445 cellular response to protein stimulus
- GO:0071548 response to dexamethasone stimulus
View more products with PI-3 KINASE specificity
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