Lipoprotein Lipase antibody | 5D2

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Mouse anti Lipoprotein Lipase:Biotin

Mouse anti Lipoprotein Lipase:HRP

Mouse anti Lipoprotein Lipase:Low Endotoxin

Mouse anti Lipoprotein Lipase

Product Type
Monoclonal Antibody
Clone
5D2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5641B E IP WB 0.1 mg
MCA5641P E IP WB 0.1 mg
MCA5641EL E FN IP WB 0.5 mg
MCA5641GA E IP WB 0.1 mg
MCA5641 E IP WB 0.2 mg
Mouse anti lipoprotein lipase antibody, clone 5D2 recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues.

A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions.

Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions.  Mouse anti Lipoprotein lipase antibody, clone 5D2 has been shown to inhibit the activity of human LPL (Chang et al. 1998).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Human
Chicken
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Purified bovine milk lipoprotein lipase.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11151 Related reagents
Entrez Gene
LPL Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004465 lipoprotein lipase activity
GO:0004620 phospholipase activity
GO:0004806 triglyceride lipase activity
GO:0006633 fatty acid biosynthetic process
GO:0006644 phospholipid metabolic process
GO:0008201 heparin binding
GO:0031225 anchored to membrane
GO:0019433 triglyceride catabolic process
GO:0034185 apolipoprotein binding
GO:0034361 very-low-density lipoprotein particle
GO:0034372 very-low-density lipoprotein particle remodeling
GO:0042627 chylomicron
Regulatory
For research purposes only

Applications of Lipoprotein Lipase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/200
Immunoprecipitation
Western Blotting
ELISA
Immunoprecipitation
Western Blotting
ELISA 1/500 1/5000
Functional Assays
Immunoprecipitation
Western Blotting
ELISA 1/500 1/5000
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
MCA5641B can be used in a direct ELISA, or as the detection reagent in a sandwich ELISA with MCA5641 or MCA5641GA as the capture antibody.
ELISA
MCA5641P can be used in a direct ELISA, or as the detection reagent in a sandwich ELISA with MCA5641 or MCA5641GA as the capture antibody.
ELISA
MCA5641EL can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA with MCA5641B or MCA5641P as the detection reagent.
ELISA
MCA5641 can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA with MCA5641B or MCA5641P as the detection reagent.
Western Blotting
MCA5641B detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
Western Blotting
MCA5641P detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
Western Blotting
MCA5641EL detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
Western Blotting
MCA5641 detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Lipoprotein Lipase antibody

  1. Peterson, J. et al. (1992) Human lipoprotein lipase: relationship of activity, heparin affinity, and conformation as studied with monoclonal antibodies.
    J Lipid Res. 33 (8): 1165-70.
  2. Chang, S.F. et al. (1998) Detailed characterization of the binding site of the lipoprotein lipase-specific monoclonal antibody 5D2.
    J Lipid Res. 39 (12): 2350-9.
  3. Hussain, M.M. et al. (2000) High affinity binding between lipoprotein lipase and lipoproteins involves multiple ionic and hydrophobic interactions, does not require enzyme activity, and is modulated by glycosaminoglycans.
    J Biol Chem. 275 (38): 29324-30.