Lipoprotein Lipase antibody | 5D2

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Anti Lipoprotein Lipase Antibody, clone 5D2 thumbnail image 1

Anti Lipoprotein Lipase Antibody, clone 5D2 thumbnail image 2

Anti Lipoprotein Lipase Antibody, clone 5D2 gallery image 1 — Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase antibody, clone 5D2 (**MCA5641**) followed by Rabbit F(ab')₂ anti Mouse IgG:HRP (STAR13B).

Anti Lipoprotein Lipase Antibody, clone 5D2 gallery image 2 — Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase:HRP (**MCA5641P**).

Mouse anti Lipoprotein Lipase:Biotin

Mouse anti Lipoprotein Lipase:HRP

Mouse anti Lipoprotein Lipase:Low Endotoxin

Mouse anti Lipoprotein Lipase

Product Type: Monoclonal Antibody
Clone: 5D2
Isotype: IgG1

Product Code	Applications	Pack Size
MCA5641B	E IP WB	0.1 mg
MCA5641P	E IP WB	0.1 mg
MCA5641EL	E FN IP WB	0.5 mg
MCA5641GA	E IP WB	0.1 mg
MCA5641	E IP WB	0.2 mg

Mouse anti lipoprotein lipase antibody, clone 5D2 recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues.

A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions.

Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein lipase antibody, clone 5D2 has been shown to inhibit the activity of human LPL (Chang et al. 1998).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat
Human
Chicken
Guinea Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.01% Thiomersal
HRP Stabiliser (BUF052A)

Preservative Stabilisers

None present

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Carrier Free

Yes

Immunogen

Purified bovine milk lipoprotein lipase.

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P11151 Related reagents
Entrez Gene: LPL Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0004465 lipoprotein lipase activity; GO:0004620 phospholipase activity; GO:0004806 triglyceride lipase activity; GO:0006633 fatty acid biosynthetic process; GO:0006644 phospholipid metabolic process; GO:0008201 heparin binding; GO:0031225 anchored to membrane; GO:0019433 triglyceride catabolic process; GO:0034185 apolipoprotein binding; GO:0034361 very-low-density lipoprotein particle; GO:0034372 very-low-density lipoprotein particle remodeling; GO:0042627 chylomicron
Regulatory: For research purposes only

Applications of Lipoprotein Lipase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/50	1/200
Immunoprecipitation
Western Blotting
ELISA
Immunoprecipitation
Western Blotting
ELISA	1/500	1/5000
Functional Assays
Immunoprecipitation
Western Blotting
ELISA	1/500	1/5000
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: MCA5641B can be used in a direct ELISA, or as the detection reagent in a sandwich ELISA with MCA5641 or MCA5641GA as the capture antibody.
ELISA: MCA5641P can be used in a direct ELISA, or as the detection reagent in a sandwich ELISA with MCA5641 or MCA5641GA as the capture antibody.
ELISA: MCA5641EL can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA with MCA5641B or MCA5641P as the detection reagent.
ELISA: MCA5641 can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA with MCA5641B or MCA5641P as the detection reagent.
Western Blotting: MCA5641B detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
Western Blotting: MCA5641P detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
Western Blotting: MCA5641EL detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
Western Blotting: MCA5641 detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Lipoprotein Lipase antibody

Peterson, J. et al. (1992) Human lipoprotein lipase: relationship of activity, heparin affinity, and conformation as studied with monoclonal antibodies.
J Lipid Res. 33 (8): 1165-70.
Chang, S.F. et al. (1998) Detailed characterization of the binding site of the lipoprotein lipase-specific monoclonal antibody 5D2.
J Lipid Res. 39 (12): 2350-9.
Hussain, M.M. et al. (2000) High affinity binding between lipoprotein lipase and lipoproteins involves multiple ionic and hydrophobic interactions, does not require enzyme activity, and is modulated by glycosaminoglycans.
J Biol Chem. 275 (38): 29324-30.

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