Lipoprotein Lipase antibody | 5D2
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E ResetMouse anti Lipoprotein Lipase
- Product Type
- Monoclonal Antibody
- Clone
- 5D2
- Isotype
- IgG1
- Specificity
- Lipoprotein Lipase
Antibody Quality and 
Unrivaled 
| Mouse anti lipoprotein lipase antibody, clone 5D2 recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues. A deficiency of LPL can result in hypertriglyceridemia, and LPL plays a critical role in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein lipase antibody, clone 5D2 inhibits the activity of human LPL (Chang et al. 1998). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Human Chicken Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified bovine milk lipoprotein lipase.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/5000 |
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Lipoprotein Lipase antibody
-
Peterson, J. et al. (1992) Human lipoprotein lipase: relationship of activity, heparin affinity, and conformation as studied with monoclonal antibodies.
J Lipid Res. 33 (8): 1165-70. -
Chang, S.F. et al. (1998) Detailed characterization of the binding site of the lipoprotein lipase-specific monoclonal antibody 5D2.
J Lipid Res. 39 (12): 2350-9. -
Hussain, M.M. et al. (2000) High affinity binding between lipoprotein lipase and lipoproteins involves multiple ionic and hydrophobic interactions, does not require enzyme activity, and is modulated by glycosaminoglycans.
J Biol Chem. 275 (38): 29324-30.
- RRID
- AB_10922752
- UniProt
- P11151
- Entrez Gene
- LPL
- GO Terms
- GO:0005886 plasma membrane
- GO:0004465 lipoprotein lipase activity
- GO:0004620 phospholipase activity
- GO:0004806 triglyceride lipase activity
- GO:0006633 fatty acid biosynthetic process
- GO:0006644 phospholipid metabolic process
- GO:0008201 heparin binding
- GO:0031225 anchored to membrane
- GO:0019433 triglyceride catabolic process
- View More GO Terms
- GO:0034185 apolipoprotein binding
- GO:0034361 very-low-density lipoprotein particle
- GO:0034372 very-low-density lipoprotein particle remodeling
- GO:0042627 chylomicron
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