Lipoprotein Lipase antibody | 5D2
Mouse anti Lipoprotein Lipase:Biotin
Mouse anti Lipoprotein Lipase:HRP
Mouse anti Lipoprotein Lipase:Low Endotoxin
Mouse anti Lipoprotein Lipase
- Product Type
- Monoclonal Antibody
- Clone
- 5D2
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA5641B | E IP WB | 0.1 mg |
|
|
| MCA5641P | E IP WB | 0.1 mg |
|
|
| MCA5641EL | E FN IP WB | 0.5 mg |
|
|
| MCA5641GA | E IP WB | 0.1 mg |
|
|
| MCA5641 | E IP WB | 0.2 mg |
|
Mouse anti lipoprotein lipase antibody, clone 5D2 recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues.
A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions.
Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein lipase antibody, clone 5D2 has been shown to inhibit the activity of human LPL (Chang et al. 1998).
A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions.
Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein lipase antibody, clone 5D2 has been shown to inhibit the activity of human LPL (Chang et al. 1998).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Human Chicken Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Purified bovine milk lipoprotein lipase.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11151
- Entrez Gene
- LPL
- GO Terms
- GO:0005886 plasma membrane
- GO:0004465 lipoprotein lipase activity
- GO:0004620 phospholipase activity
- GO:0004806 triglyceride lipase activity
- GO:0006633 fatty acid biosynthetic process
- GO:0006644 phospholipid metabolic process
- GO:0008201 heparin binding
- GO:0031225 anchored to membrane
- GO:0019433 triglyceride catabolic process
- GO:0034185 apolipoprotein binding
- GO:0034361 very-low-density lipoprotein particle
- GO:0034372 very-low-density lipoprotein particle remodeling
- GO:0042627 chylomicron
- Regulatory
- For research purposes only
Applications of Lipoprotein Lipase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50 | 1/200 | |
| Immunoprecipitation | |||
| Western Blotting | |||
| ELISA | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| ELISA | 1/500 | 1/5000 | |
| Functional Assays | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| ELISA | 1/500 | 1/5000 | |
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- MCA5641B can be used in a direct ELISA, or as the detection reagent in a sandwich ELISA with MCA5641 or MCA5641GA as the capture antibody.
- ELISA
- MCA5641P can be used in a direct ELISA, or as the detection reagent in a sandwich ELISA with MCA5641 or MCA5641GA as the capture antibody.
- ELISA
- MCA5641EL can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA with MCA5641B or MCA5641P as the detection reagent.
- ELISA
- MCA5641 can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA with MCA5641B or MCA5641P as the detection reagent.
- Western Blotting
- MCA5641B detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
- Western Blotting
- MCA5641P detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
- Western Blotting
- MCA5641EL detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
- Western Blotting
- MCA5641 detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
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Secondary Antibodies Available
Product Specific References
References for Lipoprotein Lipase antibody
-
Peterson, J. et al. (1992) Human lipoprotein lipase: relationship of activity, heparin affinity, and conformation as studied with monoclonal antibodies.
J Lipid Res. 33 (8): 1165-70. -
Chang, S.F. et al. (1998) Detailed characterization of the binding site of the lipoprotein lipase-specific monoclonal antibody 5D2.
J Lipid Res. 39 (12): 2350-9. -
Hussain, M.M. et al. (2000) High affinity binding between lipoprotein lipase and lipoproteins involves multiple ionic and hydrophobic interactions, does not require enzyme activity, and is modulated by glycosaminoglycans.
J Biol Chem. 275 (38): 29324-30.
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