Mouse anti Bovine Interleukin-10 antibody, clone CC318 recognizes bovine IL-10. Mouse anti Bovine Interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Plasmid cDNA encoding bovine IL-10.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
positive regulation of B cell apoptosis
negative regulation of B cell proliferation
negative regulation of interleukin-6 production
receptor biosynthetic process
interferon-gamma biosynthetic process
positive regulation of gene-specific transcription
negative regulation of nitric oxide biosynthetic process
negative regulation of interferon-gamma biosynthetic process
positive regulation of cytokine secretion
negative regulation of membrane protein ectodomain proteolysis
positive regulation of transcription factor activity
response to glucocorticoid stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-10 antibody
|Flow Cytometry 1
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- This reagent may be used as a capture antibody in a sandwich ELISA assay for bovine IL-10 in combination with MCA2111B as detection reagent, see Bannerman, D.D.et al.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for IL-10 antibody
Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
Vet Immunol Immunopathol. 85 (3-4): 213-23.
Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
Infect Immun. 78: 1383-9.
Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
J Leukoc Biol. 83 (1): 48-55.
Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
Int Arch Allergy Immunol. 144: 325-37.
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70.
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38.
Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
Clin Experiment Ophthalmol. 38: 405-13.
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19.
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72.
Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
Vet Res. 41: 14.
den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10.
PLoSone 6: e18188
Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
Vet Res. 44: 59.
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705.
Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.
Clin Vaccine Immunol. 17: 1946-51.
McGill, J.L. et al. (2013) Differential chemokine and cytokine production by neonatal bovine γ/δ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
Immunology. 139: 227-44.
Olivier, M. et al. (2009) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
PLoS One. 7: e30430.
Shu, D. et al. (2011) Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne's disease.
Clin Vaccine Immunol. 18: 1467-76.
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48.
Dooley LM et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
PLoS One. 10 (5): e0124144.
Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.
PLoS One. 11 (4): e0154172.
Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
Res Vet Sci. 110: 34-39.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2)Jan 26 [Epub ahead of print].
Pomeroy B et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.
Vet Immunol Immunopathol. 182: 37-42.
Davidson, J.O. et al. (2021) Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep.
Stem Cells Transl Med. 10 (3): 427-40.
Westover, A. et al. (2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
Front Physiol. 8: 871.
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7.
Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
Vet Immunol Immunopathol. 211: 44-8.
Stabel, J.R. & Bannantine, J.P. (2019) Divergent Antigen-Specific Cellular Immune Responses during Asymptomatic Subclinical and Clinical States of Disease in Cows Naturally Infected with Mycobacterium avium. subsp. paratuberculosis.
Infect Immun. 88(1):e00650-19.
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
Vet Immunol Immunopathol. 237: 110268.
Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
Front Vet Sci. 7: 9.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra