IgG2 antibody | IL-A73
Horseradish peroxidase conjugated Mouse anti Bovine IgG2 antibody, clone IL-A73 (MCA2441P) used to evaluate bovine IgG2 levels in serum samples by ELISA.
Image caption:
Antibody profile of S19 and RB51 prime vaccinated, and RB51 revaccinated cattle measured by I-ELISA using S19 and RB51 whole-cell antigens.
The results are expressed as mean. Data for total IgG, IgG1 and IgG2 are shown. Vaccinations were indicated by arrows. Lowercase letters indicate statistical difference between days on same group (one-way ANOVA followed by paired t-test).
From: Dorneles EMS, Lima GK, Teixeira-Carvalho A, Araújo MSS, Martins-Filho OA, Sriranganathan N, et al. (2015)
Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS ONE 10(9): e0136696.
doi: 10.1371/journal.pone.0136696
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine IgG2, clone IL-A73, is a monoclonal antibody specific for bovine IgG2 and does not recognise other bovine immunoglobulin classes.
While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races.
Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 (Campbell et al. 1998).
While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races.
Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 (Campbell et al. 1998).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified bovine IgG2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/1000 | |
| ELISA | 1/20 000 | 1/200 000 | |
| Flow Cytometry | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| Mouse anti Bovine IgA | MCA2438GA | E IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
|
|
| Mouse anti Bovine IgM | MCA2443GA | E F IP R | 0.1 mg |
|
|
| Mouse anti Sheep IgE | MCA5941GA | C E IF WB | 0.1 mg |
|
Product Specific References
References for IgG2 antibody
-
Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
J Leukoc Biol. 63 (5): 567-74. -
Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
Vet Immunol Immunopathol. 24 (3): 267-83. -
Williams, D.J. et al. (1996) The role of anti-variable surface glycoprotein antibody responses in bovine trypanotolerance.
Parasite Immunol. 18 (4): 209-18. -
Hecker, Y.P. et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
Vet Immunol Immunopathol. 162 (3-4): 142-53. -
Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS One. 10 (9): e0136696. -
Pereyra, R. et al. (2019) Evidence of reduced vertical transmission of Neospora caninum. associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition.
Vet Immunol Immunopathol. 208: 53-57. -
Jaramillo, J.O. et al. (2019) Immunisation of cattle against Babesia bovis. combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection.
Ticks Tick Borne Dis. 10 (6): 101270.
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