IgG1 antibody | K37 2G6
Mouse anti Bovine IgG1, clone K37 2G6, recognizes bovine IgG1 and does not cross react with other subclasses.
IgG1 along with IgG2 and IgG3 form the three major subclasses of bovine IgG. In bovids, IgG1 constitutes approximately 50% of serum immunoglobulin. IgG1. Unlike many other species, in ruminants and particularly bovids, IgG1 is major immunoglobulin present in milk and colostrum rather than IgA.
In addition to clone K37 2G6, Bio-Rad offer clone IL-A60, which is also specific to bovine IgG1, along with a wide range of other antibodies specific to various bovine immunoglobulin and immunoglobulin subclasses.
IgG1 along with IgG2 and IgG3 form the three major subclasses of bovine IgG. In bovids, IgG1 constitutes approximately 50% of serum immunoglobulin. IgG1. Unlike many other species, in ruminants and particularly bovids, IgG1 is major immunoglobulin present in milk and colostrum rather than IgA.
In addition to clone K37 2G6, Bio-Rad offer clone IL-A60, which is also specific to bovine IgG1, along with a wide range of other antibodies specific to various bovine immunoglobulin and immunoglobulin subclasses.
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified Bovine IgG1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG1:HRP | MCA2440P | E | 0.25 mg |
|
Product Specific References
References for IgG1 antibody
-
Estes. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
Immunology. 95 (4): 604- 611 -
Soria, I. et al. (2018) Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle.
J Immunol Res. 2018: 3497401. -
McNeilly, T.N. et al. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge.
Vaccine. 28 (5): 1422-8. -
Salem, E. et al. (2019) Pathogenesis, Host Innate Immune Response, and Aerosol Transmission of Influenza D Virus in Cattle.
J Virol. 93(7):e01853-18. -
McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling.
Vaccine. 28 (5): 1412-21.
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