GFAP antibody | 2E1
Mouse anti Bovine GFAP antibody, clone 2E1 (MCA4734) used to evaluate GFAP expression in mouse brain by immunofluorescence
Image caption:
Immunofluorescence staining for assessment of an astrocyte marker GFAP in the corpus callosum in mice receiving cuprizone for induction of demyelination and further treatment with L-a-aminoadipate (L-AAA). Cuprizone exposure caused an apparent increase in the level of protein expression for GFAP indicative of astrogloisis in the animals. Administration of L-AAA to induce astrocyte ablation could cause an observable reduction in the rate expression for GFAP. Arrows are indicative of GFAP+ cells. Quantification of immunofluorescence data (Graph e) revealed that the alterations in the rate of expression for GFAP in the understudying groups were significant. Scale bars: 50 μm. (n = 3 per group) ***p <0.001 (ANOVA followed by post-hoc analysis)
From: Madadi S, Pasbakhsh P, Tahmasebi F, Mortezaee K, Khanehzad M, Boroujeni FB, Noorzehi G, Kashani IR.
Astrocyte ablation induced by La-aminoadipate (L-AAA) potentiates remyelination in a cuprizone demyelinating mouse model.
Metab Brain Dis. 2019 Apr;34(2):593-603.
doi: 10.1007/s11011-019-0385-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine GFAP
- Product Type
- Monoclonal Antibody
- Clone
- 2E1
- Isotype
- IgG2b
- Specificity
- GFAP
Antibody Quality and Performance Guarantee
| Mouse anti Bovine GFAP antibody, clone 2E1 recognizes glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death. Mouse anti Bovine GFAP antibody, clone 2E1 may be used in conjunction with Mouse anti Bovine GFAP antibody, clone 1B4 (MCA4733) and Mouse anti Bovine GFAP antibody, clone 4A11 (MCA4684) for increased sensitivity when used in immunohistology. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Mouse Rat Sheep Dog Pig Rabbit Guinea Pig Chicken - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine spinal cord homogenate.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunohistology - Paraffin 1 | |
||
| Radioimmunoassays | |
||
| Western Blotting | |
1/200 | 1/2000 |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Brain
References for GFAP antibody
-
Pegram, C.N. et al. (1985) Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.
Neurochem Pathol. 3 (2): 119-38. -
McLendon, R.E. et al. (1986) The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues.
J Neuropathol Exp Neurol. 45 (6): 692-703. -
Madadi, S. et al. (2019) Astrocyte ablation induced by La-aminoadipate (L-AAA) potentiates remyelination in a cuprizone demyelinating mouse model.
Metab Brain Dis. 34 (2): 593-603.
- RRID
- AB_10567987
- UniProt
- P14136
- P47819
- P03995
- Q28115
- Entrez Gene
- Gfap
- GFAP
- Gfap
- GFAP
- GO Terms
- GO:0005882 intermediate filament
- GO:0005200 structural constituent of cytoskeleton
- GO:0005737 cytoplasm
- GO:0009611 response to wounding
MCA4734GA
152849
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