CD86 antibody | IL-A190
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Mouse anti Bovine CD86
- Product Type
- Monoclonal Antibody
|Mouse anti Bovine CD86 antibody, cllone IL-A190 recognises the bovine CD86 cell surface antigen, which is expressed by dendritic cells, activated macrophages and activated B cells. CD86 plays an important role in co-stimulation of T cells in the primary immune response.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD86 antibody
Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
Immunology. 108: 55-61.
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84 (Pt 7): 1771-80.
Rhodes, S.G. et al. (2003) 1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.
Clin Diagn Lab Immunol. 10 (6): 1129-35.
Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
J Leukoc Biol. 76 (3): 553-61.
Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
Scand J Immunol. 61: 242-50
Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node.
J Leukoc Biol. 79: 268-76.
Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
J Immunol. 180: 5963-72.
Hemati, B. et al. (2009) Bluetongue virus targets conventional dendritic cells in skin lymph.
J Virol. 83: 8789-99.
View The Latest Product References
Ruscanu, S. et al. (2012) The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
J Virol. 2012 May;86: 5817-28.
Mauro, A. et al. (2016) M1 and M2 macrophage recruitment during tendon regeneration induced by amniotic epithelial cell allotransplantation in ovine.
Res Vet Sci. 105: 92-102.
Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
BMC Vet Res. 13 (1): 390.
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365.
Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
Vet Immunol Immunopathol. 19 Nov: 110363.
Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
PLoS One. 17 (12): e0278313.
Russo, V. et al. (2022) Tendon Healing Response Is Dependent on Epithelial-Mesenchymal-Tendon Transition State of Amniotic Epithelial Stem Cells.
Biomedicines. 10 (5): 1177.
Wherry, T.L.T. et al. (2022) Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.
Front Vet Sci. 9: 830144.
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