CD86 antibody | IL-A190
Mouse anti Rat CD86 antibody, clone 24F (MCA2874GA) used to label CD86 expressing cells in rat tendons by immunofluorescence.
Image caption:
Percentage of M1 and M2 macrophage markers and gene expression of interleukins IL12 and IL10 in explanted tendons. (B) Representative images of CD86- and (C) CD206-positive cells (green fluorescence) in CTR and tdAEC-treated tendons, taken as an example, at day 14 and 28. Nuclei were counterstained with DAPI (blue fluorescence). Scale bar: 50 μm. (D) Quantification of CD86- and CD206-positive cells related to M1 and M2 macrophage phenotypes, respectively, in the defect area (from area 1 to area 5) of healing tendons performed at 14 and 28 days post-treatment. a Statistically significant vs. CTR (p <0.05), b statistically significant vs. eAECs (p <0.05), c statistically significant vs. mAECs (p <0.05), and d statistically significant vs. tdAECs (p <0.05).
From: Russo V, Mauro A, Peserico A, Di Giacinto O, Khatib ME, Citeroni MR, Rossi E, Canciello A, Mazzotti E, Barboni B.
Tendon Healing Response Is Dependent on Epithelial-Mesenchymal-Tendon Transition State of Amniotic Epithelial Stem Cells.
Biomedicines. 2022 May 19;10(5):1177.
doi: 10.3390/biomedicines10051177.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Alexa Fluor 647 conjugated Mouse anti Rat CD86 antibody, clone 24F (MCA2874A647) used to identify rat dentritic cells by flow cytometry.
Image caption:
Representative gating images of flow cytometry analysis for immune cell populations using myeloid cell marker CD11b, granulocyte marker HIS48, T cell marker CD3/CD4/CD8, B cell marker CD45RA, NK cell marker CD161, and dendritic cell marker CD86.
From: Zhou X, Zhang C, Wu X, Hu X, Zhang Y, Wang X, Zheng L, Gao P, Du J, Zheng W, Shang H, Hu K, Jiang Z, Nie Y, Hu S, Xiao RP, Zhu X, Xiong JW.
Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.
Nat Commun. 2022 Nov 5;13(1):6672.
doi: 10.1038/s41467-022-33631-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD86
- Product Type
- Monoclonal Antibody
- Clone
- IL-A190
- Isotype
- IgG1
- Specificity
- CD86
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD86 antibody, cllone IL-A190 recognizes the bovine CD86 cell surface antigen, expressed by dendritic cells, activated macrophages and activated B cells. CD86 plays an important role in co-stimulation of T cells in the primary immune response. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD86 antibody
-
Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
Immunology. 108: 55-61. -
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84 (Pt 7): 1771-80. -
Rhodes, S.G. et al. (2003) 1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.
Clin Diagn Lab Immunol. 10 (6): 1129-35. -
Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
J Leukoc Biol. 76 (3): 553-61. -
Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
Scand J Immunol. 61: 242-50 -
Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node.
J Leukoc Biol. 79: 268-76. -
Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
J Immunol. 180: 5963-72. -
Hemati, B. et al. (2009) Bluetongue virus targets conventional dendritic cells in skin lymph.
J Virol. 83: 8789-99.
View The Latest Product References
-
Ruscanu, S. et al. (2012) The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
J Virol. 2012 May;86: 5817-28. -
Mauro, A. et al. (2016) M1 and M2 macrophage recruitment during tendon regeneration induced by amniotic epithelial cell allotransplantation in ovine.
Res Vet Sci. 105: 92-102. -
Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
BMC Vet Res. 13 (1): 390. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
Vet Immunol Immunopathol. 19 Nov: 110363. -
Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
PLoS One. 17 (12): e0278313. -
Russo, V. et al. (2022) Tendon Healing Response Is Dependent on Epithelial-Mesenchymal-Tendon Transition State of Amniotic Epithelial Stem Cells.
Biomedicines. 10 (5): 1177. -
Wherry, T.L.T. et al. (2022) Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.
Front Vet Sci. 9: 830144. -
Zenobi, M.G. et al. (2020) Effect of prepartum energy intake and supplementation with ruminally protected choline on innate and adaptive immunity of multiparous Holstein cows.
J Dairy Sci. 103 (3): 2200-16. -
Zhou, X. et al. (2022) Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.
Nat Commun. 13 (1): 6672.
- UniProt
- Q1JPC5
MCA2437GA
163078
164702
1708
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD86 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Bovine ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up