CD86 antibody | IL-A190
Filter by Application:
F ResetMouse anti Bovine CD86
- Product Type
- Monoclonal Antibody
- Clone
- IL-A190
- Isotype
- IgG1
- Specificity
- CD86
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Bovine CD86 antibody, cllone IL-A190 recognises the bovine CD86 cell surface antigen, which is expressed by dendritic cells, activated macrophages and activated B cells. CD86 plays an important role in co-stimulation of T cells in the primary immune response. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD86 antibody
-
Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
Scand J Immunol. 61: 242-50 -
Rhodes, S.G. et al. (2003) 1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.
Clin Diagn Lab Immunol. 10 (6): 1129-35. -
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84 (Pt 7): 1771-80. -
Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
J Leukoc Biol. 76 (3): 553-61. -
Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node.
J Leukoc Biol. 79: 268-76. -
Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
Immunology. 108: 55-61. -
Hemati, B. et al. (2009) Bluetongue virus targets conventional dendritic cells in skin lymph.
J Virol. 83: 8789-99. -
Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
J Immunol. 180: 5963-72.
View The Latest Product References
-
Ruscanu, S. et al. (2012) The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway.
J Virol. 2012 May;86: 5817-28. -
Mauro, A. et al. (2016) M1 and M2 macrophage recruitment during tendon regeneration induced by amniotic epithelial cell allotransplantation in ovine.
Res Vet Sci. 105: 92-102. -
Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
BMC Vet Res. 13 (1): 390. -
Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
Vet Immunol Immunopathol. 19 Nov: 110363. -
Wherry, T.L.T. et al. (2022) Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.
Front Vet Sci. 9: 830144. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Russo, V. et al. (2022) Tendon Healing Response Is Dependent on Epithelial-Mesenchymal-Tendon Transition State of Amniotic Epithelial Stem Cells.
Biomedicines. 10 (5): 1177.
- UniProt
- Q1JPC5
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