CD4 antibody | CC8
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the evaluation of CD4 expression on proliferating bovine peripheral blood mononuclear cells by flow cytometry.
Image caption:
Determination of phenotypes of proliferating PBMC by FACS analysis. Determination of phenotypes (CD4+, CD8+ and WC1+) of proliferating PBMC by CFDA SE staining after re-stimulating with respective vaccine antigen and fluorescence-activated cell sorter (FACS) analysis. PBMC were separated from heparinised blood of vaccinated cattle originated from full dose vaccine group on 4 weeks after homologous FMDV challenge. Two naïve cattle without FMDV infection and two A Malaysia 97 FMDV infected cattle (4 weeks post-infection) without prior vaccination were included as control group. PBMCs from naïve animals were re-stimulated with SAT2 Eritrea vaccine antigen. FACS plots were exemplified only for one vaccinated challenged animal from SAT2 Eritrea animal experiment. Similar FACS plots were obtained for the PBMC originated from A Malaysia 97 and SAT2 Eritrea vaccinated animals. Error bars represent standard error of the mean.
From: Oh Y, Fleming L, Statham B, Hamblin P, Barnett P, Paton DJ, et al. (2012)
Interferon-γ Induced by In Vitro Re-Stimulation of CD4+ T-Cells Correlates with In Vivo FMD Vaccine Induced Protection of Cattle against Disease and Persistent Infection.
PLoS ONE 7(9): e44365.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of CD4 expressing lymphocytes in preipheral blood by flow cytometry.
Image caption:
Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN-γ within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN-γ and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.
From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of CD4 expressing lymphocytes in preipheral blood by flow cytometry.
Image caption:
Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN-γ, IL-2 and TNF-α determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN-γ, IL-2 or TNF-α following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN-γ+ and IFN-γ− CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle.
From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
APC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653APC) used for the identification of CD4 expressing T-cells in bovine peripheral blood by flow cytoometry together with RPE conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to detect cytotoxic T-cells in the same samples.
Image caption:
Effects of supplementation with rumen-protected choline (TC), rumen-protected methionine (TM) or both (TCM) on proportions of T lymphocyte subtypes in peripheral blood of transition dairy cows. (A) and (B) present the output images from flow cytometry. (C) displays the effects of each treatment over the three weeks before calving, on calving day, and over the three weeks after calving. a, b, c Values with different superscript letters at the same time point are significantly different (P <0.05).
From: Sun F, Cao Y, Cai C, Li S, Yu C, Yao J (2016)
Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS ONE 11(8): e0160659.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653GA) used to evaluate CD4 expression on lymphocyte subsets from bovine peripheral blood by flow cytometry.
Image caption:
Proliferation of bovine lymphocytes and major T-cell subsets in the presence of various DON concentrations. PBMCs were stained with violet proliferation dye and cultivated in vitro in the presence of ConA and various DON concentrations for four days. After harvest, major T-cell subsets were labeled by antibodies against CD8β, CD4, and T-cell receptor (TCR)-γδ and analyzed by flow cytometry. (a) Lymphocytes were gated by light scatter properties and further subgated for the expression of CD8β, CD4, and TCR-γδ (pseudocolor plots). Histograms show the fluorescence intensities of the violet proliferation dye in lymphocytes (top) and the identified T-cell subsets under different cultivation conditions. Solid horizontal lines indicate the parental generation (on the right) and the proliferating generations (on the left). Numbers located in the two upper corners of the histograms indicate the frequency in % of the proliferating populations in comparison to the nonproliferating cells. Representative data from one animal out of six are shown. SSC-A side scatter area, FSC-A: forward scatter area.
From: Novak B, Vatzia E, Springler A, Pierron A, Gerner W, Reisinger N, Hessenberger S, Schatzmayr G, Mayer E.
Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine.
Toxins (Basel). 2018 Apr 11;10(4). pii: E152.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Alexa Fluor™647 conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653A647) used to assess CD4 expression on bovine lymphocytes by flow cytometry.
Image caption:
Identification of IL-17A and IFN-γ producing bovine lymphocytes. PBMC were stimulated with PMA/ionomycin, cytokine secretion blocked with Brefeldin A before flow cytometry analysis. Debris were excluded by gating according to FSC/SSC, and after gating on singlet cells, dead cells were excluded by gating on live cells. The CD4+ cells were gated by taking into account the isotype control, and the production of IL-17A and IFN-γ was measured by intracellular labelling with specific antibodies. Results shown are from a representative experiment. FSC: forward scatter: SSC: side scatter.
From: Cunha P, Vern YL, Gitton C, Germon P, Foucras G, Rainard P.
Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Sci Rep. 2019 Nov 6;9(1):16115.
doi: 10.1038/s41598-019-52562-2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD4:Alexa Fluor® 647
Mouse anti Bovine CD4:FITC
Mouse anti Bovine CD4
Mouse anti Bovine CD4:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CC8
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1653A647 | F | 100 Tests/1ml |
|
|
| MCA1653F | F | 0.1 mg |
|
|
| MCA1653GA | C F IP | 0.1 mg |
|
|
| MCA1653PE | F | 100 Tests |
|
Mouse anti Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskra et al. 1991).
A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324 T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori, et al.. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori, et al.. 2020).
A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324 T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori, et al.. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori, et al.. 2020).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Bovine lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunized mouse were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- A7YY52
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
|
Product Specific References
References for CD4 antibody
-
Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).
Vet Immunol Immunopathol. 27 (1-3): 51-4. -
Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
Vet Immunol Immunopathol. 27 (1-3): 215-22. -
Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
Vet Immunol Immunopathol. 27 (1-3): 21-7. -
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. -
Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
Infect Immun. 76: 3932-9. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Buddle, B.M. et al. (2003) Revaccination of neonatal calves with Mycobacterium bovis BCG reduces the level of protection against bovine tuberculosis induced by a single vaccination.
Infect Immun. 71: 6411-9. -
Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.
J Virol. 83: 4039-50. -
Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells
Immunology. 105: 190-5. -
Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
BMC Vet Res. 6: 39. -
Hu, X.D. et al. (2009) Immunotherapy with combined DNA vaccines is an effective treatment for M. bovis infection in cattle
Vaccine. 27: 1317-22. -
Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
Vet Res. 41: 14. -
Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194. -
Wernike, K. et al. (2013) Oral exposure, reinfection and cellular immunity to Schmallenberg virus in cattle.
Vet Microbiol. pii: S0378-1135(13)00092-8. -
Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
Vet Res Commun. 34: 197-203. -
Dacal,. V. et al. (2013) Immunohistochemical characterization of inflammatory cells in the skin of cattle undergoing repeated infestations with Hypoderma lineatum (Diptera: Oestridae) larvae.
J Comp Pathol. 145: 282-8. -
Oh, Y. et al. (2012) Interferon-γ induced by in vitro re-stimulation of CD4+ T-cells correlates with in vivo FMD vaccine induced protection of cattle against disease and persistent infection.
PLoS One. 7: e44365. -
Hine, B.C. et al. (2012) Analysis of leukocyte populations in Canadian Holsteins classified as high or low immune responders for antibody- or cell-mediated immune response.
Can J Vet Res. 76: 149-56. -
Aranday-Cortes, E. et al. (2012) Transcriptional profiling of disease-induced host responses in bovine tuberculosis and the identification of potential diagnostic biomarkers.
PLoS One. 7: e30626. -
Tenaya, I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
Vet Immunol Immunopathol. 149: 167-76. -
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10. -
Blunt, L. et al. (2015) Phenotypic characterization of bovine memory cells responding to mycobacteria in IFN&gama; enzyme linked immunospot assays.
Vaccine. 33 (51): 7276-82. -
Grit, G.H. et al. (2014) Evaluation of cellular and humoral systemic immune response against Giardia duodenalis infection in cattle.
Vet Parasitol. 202 (3-4): 145-55. -
Diaz-San Segundo, F. et al. (2016) Combination of Adt-O1Manisa and Ad5-boIFNλ3 induces early protective immunity against foot-and-mouth disease in cattle.
Virology. 499: 340-9. -
Okagawa, T. et al. (2016) Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle.
Infect Immun. 84 (10): 2779-90. -
Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Dev Comp Immunol. 27 (10): 889-97. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441. -
Novak, B. et al. (2018) Bovine Peripheral Blood Mononuclear Cells Are More Sensitive to Deoxynivalenol Than Those Derived from Poultry and Swine.
Toxins (Basel). 10 (4)Apr 11 [Epub ahead of print]. -
Benedictus, L. et al. (2019) Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk.
BMC Vet Res. 15 (1): 15. -
Cunha, P. et al. (2019) Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Sci Rep. 9 (1): 16115. -
Grandoni, F. et al. (2019) A new polymorphic epitope of bovine CD4 antigen evidenced by flow cytometry.
Vet Immunol Immunopathol. 219: 109957. -
Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. Nov 06 [Epub ahead of print]. -
Metcalfe, H.J. et al. (2016) Protection associated with a TB vaccine is linked to increased frequency of Ag85A-specific CD4(+) T cells but no increase in avidity for Ag85A.
Vaccine. 34 (38): 4520-5. -
Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
Infect Immun. 88 (3) Feb 20 [Epub ahead of print]. -
Sun, F. et al. (2016) Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS One. 11 (8): e0160659. -
Kato-Mori, Y. et al. (2020) Characterization of a variant CD4 molecule in Japanese Black cattle
Vet Immunol and Immunopathol. 2020: 110167 [Epub ahead of print]. -
de Araújo, F.A. et al. (2019) Distinct immune response profile during Rhipicephalus (Boophilus) microplus infestations of guzerat dairy herd according to the maternal lineage ancestry (mitochondrial DNA).
Vet Parasitol. 273: 36-44. -
Denholm, S.J. et al. (2017) Estimating genetic and phenotypic parameters of cellular immune-associated traits in dairy cows.
J Dairy Sci. 100 (4): 2850-62. -
Bassi, P.B. et al. (2018) Parasitological and immunological evaluation of cattle experimentally infected with Trypanosoma vivax.
Exp Parasitol. 185: 98-106. -
Nakajima, N. et al. (2019) Effects of direct exposure to cold weather under grazing in winter on the physiological, immunological, and behavioral conditions of Japanese Black beef cattle in central Japan.
Anim Sci J. 90 (8): 1033-41.
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