CD28 antibody | CC220


For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Plasmid DNA encoding the bovine CD28 gene.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only (ship +4oC)
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q28071
- Entrez Gene
- CD28
- GO Terms
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0042102 positive regulation of T cell proliferation
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045840 positive regulation of mitosis
- Regulatory
- For research purposes only
Applications of CD28 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Functional Assays | 5 | ug/ml | |
Flow Cytometry | |||
Functional Assays |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
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|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Product Specific References
References for CD28 antibody
-
Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
Vet Immunol Immunopathol. 140 (1-2): 47-54. -
Goto, S. et al. (2017) Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Immun Inflamm Dis. 5 (3): 355-363. -
Nishimori, A. et al. (2017) In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.
PLoS One. 12 (4): e0174916. -
Cunha, P. et al. (2019) Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Sci Rep. 9 (1): 16115.
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