CD28 antibody | CC220
Mouse anti Bovine CD28 antibody, clone CC220 (MCA5778EL) used in vitro to evaluate the effect on interferon γ production by peripheral blood derived mononuclear cells from healthy and mycoplasmosis affected cattle
Image caption:
IFN‐γ production in bovine mycoplasmosis. IFN‐γ production was determined in supernatant of the PBMCs culture from mycoplasmosis‐afflicted and healthy control Holstein cattle by ELISA assay. PBMCs were incubated with the combination of anti‐bovine CD3 and anti‐bovine CD28 antibodies. Each line indicates the median production in each group.
From: Goto S, Konnai S, Okagawa T, Nishimori A, Maekawa N, Gondaira S, Higuchi H, Koiwa M, Tajima M, Kohara J, Ogasawara S, Kato Y, Suzuki Y, Murata S, Ohashi K.
Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Immun Inflamm Dis. 2017 Sep;5(3):355-63.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD28 antibody, clone CC220 (MCA5778EL) used for the in vitro stimulation of bovine PBMCs for the subsequent evaluation of interferon γ production.
Image caption:
Activation of T-cell proliferation and interferon-γ (IFN-γ) production by Boch4G12.
(A and B) The proliferation of CD4+ and CD8+ cells in purified bovine CD3+ T cells. Peripheral blood mononuclear cells (PBMCs) isolated from healthy cattle were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE), stimulated with 1 μg/ml anti-CD3 and anti-CD28 antibodies, and then cultured for 3 days with enhanced green fluorescent protein (EGFP) cells or programmed death-ligand 1 (PD-L1)/EGFP cells without antibodies (A), or PD-L1/EGFP cells in the presence of 10 μg/ml bovine IgG control or Boch4G12 (B). n = 10 and 6, respectively; A P-value less than 0.05 was considered statistically significant. *, P <0.05, paired t-test. (C) IFN-γ production in the culture supernatant of PBMCs from bovine leukemia virus (BLV)-infected cattle (n = 10, AL:5, PL:5). PBMCs were stimulated with the supernatant of FLK-BLV cells and cultured for 6 days in the presence of 10 μg/ml rat IgG control, 4G12, bovine IgG control or Boch4G12. The concentration of IFN-γ in the culture supernatants was measured using ELISA. A P-value less than 0.05 was considered statistically significant. **, P <0.01, Wilcoxon signed rank test. Data are the means of three replicate experiment
From: Nishimori A, Konnai S, Okagawa T, Maekawa N, Ikebuchi R, Goto S, et al. (2017)
In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.
PLoS ONE 12(4): e0174916.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD28, clone CC220 recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Mouse anti Bovine CD28, clone CC220 is able to stimulate bovine T lymphocytes in combination with anti-bovine CD3 (Nishimori et al. 2017).
For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014).
For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Plasmid DNA encoding the bovine CD28 gene.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only (ship +4oC)
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q28071
- Entrez Gene
- CD28
- GO Terms
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0042102 positive regulation of T cell proliferation
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045840 positive regulation of mitosis
- Regulatory
- For research purposes only
Applications of CD28 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Functional Assays | 5 | ug/ml | |
| Flow Cytometry | |||
| Functional Assays |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD28 antibody
-
Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
Vet Immunol Immunopathol. 140 (1-2): 47-54. -
Goto, S. et al. (2017) Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Immun Inflamm Dis. 5 (3): 355-363. -
Nishimori, A. et al. (2017) In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.
PLoS One. 12 (4): e0174916. -
Cunha, P. et al. (2019) Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Sci Rep. 9 (1): 16115.
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