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CD25 antibody | IL-A111

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Anti Bovine CD25 Antibody, clone IL-A111 thumbnail image 1 Anti Bovine CD25 Antibody, clone IL-A111 thumbnail image 2 Anti Bovine CD25 Antibody, clone IL-A111 thumbnail image 3 Anti Bovine CD25 Antibody, clone IL-A111 thumbnail image 4
Anti Bovine CD25 Antibody, clone IL-A111 gallery image 1
Published customer image:
Mouse anti Bovine CD25 antibody, clone IL-A111 (MCA2430) used to evaluate CD25 expression on NKp46 expressing cells following in vitro culture by flow cytometry.
Image caption:
Phenotype of cultured NKp46+ populations. Ovine NKp46+ populations after 7-8 days of in vitro culture were stained with monoclonal antibodies against CD2, CD8, CD16 and CD25 (solid lines) or isotype controls (broken lines). Results are shown from one culture that was representative of those derived from five animals.

From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine CD25 Antibody, clone IL-A111 gallery image 2
Figure A. FITC conjugated mouse anti bovine CD25 (MCA2430F) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated mouse anti bovine CD25 (MCA2430F) and RPE conjugated mouse anti bovine CD4 (MCA1653PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD25 Antibody, clone IL-A111 gallery image 3
Figure A. RPE conjugated mouse anti bovine CD4 (MCA1653PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD4 (MCA1653PE) and FITC conjugated mouse anti bovine CD25 (MCA2430F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD25 Antibody, clone IL-A111 gallery image 4
Published customer image:
FITC conjugated Mouse anti Bovine CD25 antibody, clone IL-A111 (MCA2430F) used to evaluate CD25 expression on bovine lymphocytes by flow cytometry.
Image caption:
Gating strategy used to quantify CD25 expression on in vitro re-stimulated CD4+CD45RO+ and CD8αhiCD45RO+ cells by flow cytometry. Representative dot plots obtained with PBMC after 5 days of in vitro cultivation with Concanavalin A and subsequent tri-colour immunolabelling. A In a first analytical step, the non-blast cell (Region R0) and blast cell population (R1) were acquired for gating based on its forward versus side scatter (FSC/SSC) characteristics. B In samples labelled with anti-bovine CD4 antibodies, CD4+ cells within the blast cell population were defined by R2 as such that less than 2% of events were considered positive in the corresponding isotype control sample. C In samples labelled with anti-bovine CD8α antibodies, CD8αhi cells were defined by R3, thereby excluding the majority of CD8αlo cells. D Cells within R2 (CD4+) and R3 (CD8αhi) were displayed on CD45RO/CD25 dot plots and the geometric mean for detection of CD25 expression was assessed for all CD45RO+ within the CD4+ and CD8αhi, respectively, as defined by R4 (shaded area).

From: Schmidt N, Barth SA, Frahm J, Meyer U, Dänicke S, Geue L, Menge C.
Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 2018 Mar 7;49(1):28.
doi: 10.1186/s13567-018-0523-0.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine CD25:FITC

Mouse anti Bovine CD25

Mouse anti Bovine CD25:RPE

Product Type
Monoclonal Antibody
Clone
IL-A111
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2430F
Datasheet
F
MSDS
0.1 mg loader

MCA2430GA
Datasheet
C F IP
MSDS
0.1 mg loader

MCA2430PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells.

Mouse anti Bovine CD25 antibody, clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessens et al. 1992).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
P12342
Entrez Gene
IL2RA
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
Regulatory
For research purposes only

Applications of CD25 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD25 antibody

  1. Naessens, J. et al. (1992) Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene.
    Immunology. 76 (2): 305-9.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  3. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
    J Immunol. 159 (11): 5372-82.
  4. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  5. Evans, C.W. et al. (1994) Antigen recognition and activation of ovine gamma delta T cells.
    Immunology. 82 (2): 229-37.
  6. Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
    J Leukoc Biol. 63 (5): 567-74.
  7. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  8. Menge, C. et al. (2004) Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection.
    J Med Microbiol. 53: 573-9.
  9. Rhodes, S.G. et al. (1999) Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.
    J Gen Virol. 80 : 1673-9.
  10. Piper, E.K. et al. (2009) Immunological profiles of Bos taurus and Bos indicus cattle infested with the cattle tick, Rhipicephalus (Boophilus) microplus.
    Clin Vaccine Immunol. 16: 1074-86.
  11. Woolums, A.R. et al. (2013) Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves.
    Am J Vet Res. 74: 343-54.
  12. Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
    Immunology. 96 (3): 396-403.
  13. Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
    Pol J Vet Sci. 15: 11-20.
  14. Menge, C. et al. (2003) Verotoxin 1 from Escherichia coli affects Gb3/CD77+ bovine lymphocytes independent of interleukin-2, tumor necrosis factor-alpha, and interferon-alpha.
    Exp Biol Med (Maywood). 228: 377-86.
  15. Menge, C. et al. (1999) Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro.
    Infect Immun. 67: 2209-17.
  16. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
    Int J Parasitol. 40: 865-75.
  17. Maślanska, T. and Jaroszewski, J.J. (2013) In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25-CD4+ cells.
    J Vet Sci. 14 (2): 125-34.
  18. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82 (3): 403-10.
  19. Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
    Dev Comp Immunol. 47 (2): 254-63.
  20. Kang, H.J. et al. (2016) Effects of Ambient Temperature on Growth Performance, Blood Metabolites, and Immune Cell Populations in Korean Cattle Steers.
    Asian-Australas J Anim Sci. 29 (3): 436-43.
  21. Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
    Vet Res. 49 (1): 28.
  22. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
    Vet Immunol Immunopathol. 202: 74-84.
  23. Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. Nov 06 [Epub ahead of print].
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