CD25 antibody | IL-A111
Mouse anti Bovine CD25 antibody used to evaluate CD25 expression on NKp46 expressing cells following in vitro culture by flow cytometry.
Image caption:
Phenotype of cultured NKp46+ populations. Ovine NKp46+ populations after 7-8 days of in vitro culture were stained with monoclonal antibodies against CD2, CD8, CD16 and CD25 (solid lines) or isotype controls (broken lines). Results are shown from one culture that was representative of those derived from five animals.
From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells.
Clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessens et al. 1992).
Clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessens et al. 1992).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P12342 Related reagents
- Entrez Gene
- IL2RA Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- Regulatory
- For research purposes only
Applications of CD25 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Product Specific References
References for CD25 antibody
-
Naessens, J. et al. (1992) Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene.
Immunology. 76 (2): 305-9. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J Immunol. 159 (11): 5372-82. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Evans, C.W. et al. (1994) Antigen recognition and activation of ovine gamma delta T cells.
Immunology. 82 (2): 229-37. -
Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
J Leukoc Biol. 63 (5): 567-74. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Menge, C. et al. (2004) Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection.
J Med Microbiol. 53: 573-9. -
Rhodes, S.G. et al. (1999) Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.
J Gen Virol. 80 : 1673-9. -
Piper, E.K. et al. (2009) Immunological profiles of Bos taurus and Bos indicus cattle infested with the cattle tick, Rhipicephalus (Boophilus) microplus.
Clin Vaccine Immunol. 16: 1074-86. -
Woolums, A.R. et al. (2013) Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves.
Am J Vet Res. 74: 343-54. -
Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
Immunology. 96 (3): 396-403. -
Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
Pol J Vet Sci. 15: 11-20. -
Menge, C. et al. (2003) Verotoxin 1 from Escherichia coli affects Gb3/CD77+ bovine lymphocytes independent of interleukin-2, tumor necrosis factor-alpha, and interferon-alpha.
Exp Biol Med (Maywood). 228: 377-86. -
Menge, C. et al. (1999) Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro.
Infect Immun. 67: 2209-17. -
Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75. -
Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
Pol J Vet Sci. 15 (1): 11-20. -
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10. -
Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
Dev Comp Immunol. 47 (2): 254-63. -
Kang, H.J. et al. (2016) Effects of Ambient Temperature on Growth Performance, Blood Metabolites, and Immune Cell Populations in Korean Cattle Steers.
Asian-Australas J Anim Sci. 29 (3): 436-43. -
Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 49 (1): 28.
