CD25 antibody | IL-A111
Mouse anti Bovine CD25 antibody, clone IL-A111 (MCA2430) used to evaluate CD25 expression on NKp46 expressing cells following in vitro culture by flow cytometry.
Image caption:
Phenotype of cultured NKp46+ populations. Ovine NKp46+ populations after 7-8 days of in vitro culture were stained with monoclonal antibodies against CD2, CD8, CD16 and CD25 (solid lines) or isotype controls (broken lines). Results are shown from one culture that was representative of those derived from five animals.
From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Bovine CD25 antibody, clone IL-A111 (MCA2430F) used to evaluate CD25 expression on bovine lymphocytes by flow cytometry.
Image caption:
Gating strategy used to quantify CD25 expression on in vitro re-stimulated CD4+CD45RO+ and CD8αhiCD45RO+ cells by flow cytometry. Representative dot plots obtained with PBMC after 5 days of in vitro cultivation with Concanavalin A and subsequent tri-colour immunolabelling. A In a first analytical step, the non-blast cell (Region R0) and blast cell population (R1) were acquired for gating based on its forward versus side scatter (FSC/SSC) characteristics. B In samples labelled with anti-bovine CD4 antibodies, CD4+ cells within the blast cell population were defined by R2 as such that less than 2% of events were considered positive in the corresponding isotype control sample. C In samples labelled with anti-bovine CD8α antibodies, CD8αhi cells were defined by R3, thereby excluding the majority of CD8αlo cells. D Cells within R2 (CD4+) and R3 (CD8αhi) were displayed on CD45RO/CD25 dot plots and the geometric mean for detection of CD25 expression was assessed for all CD45RO+ within the CD4+ and CD8αhi, respectively, as defined by R4 (shaded area).
From: Schmidt N, Barth SA, Frahm J, Meyer U, Dänicke S, Geue L, Menge C.
Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 2018 Mar 7;49(1):28.
doi: 10.1186/s13567-018-0523-0.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Fitc and RPE conjugated Mouse anti Bovine CD25 antibody, clone IL-A111 (MCA2430F and MCA2430PE) used to assess CD25 expression on bovine cells by flow cytometry.
Image caption:
CD25 (IL-2R) expression assessed by flow cytometry in infected co-cultures (MOI=1) of cells obtained from five different calves (N=5). For each animal sample and condition tested a single cell well was analyzed. The results are expressed as CD25+ cells among total WC1+ (A) and NKp46+ (B) populations. Whiskers and plots indicate minimum, maximum and mean values. *p<0.05 using Friedman test with Dunn’s post- test for multiple comparisons.
From: Blanco FC, Gravisaco MJ, Bigi MM, García EA, Marquez C, McNeil M, Jackson M, Bigi F.
Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells.
Front Immunol. 2021 Jul 15;12:674643.
doi: 10.3389/fimmu.2021.674643.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD25
- Product Type
- Monoclonal Antibody
- Clone
- IL-A111
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells. Mouse anti Bovine CD25 antibody, clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessens et al. 1992). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD25 antibody
-
Naessens, J. et al. (1992) Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene.
Immunology. 76 (2): 305-9. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J Immunol. 159 (11): 5372-82. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Evans, C.W. et al. (1994) Antigen recognition and activation of ovine gamma delta T cells.
Immunology. 82 (2): 229-37. -
Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
J Leukoc Biol. 63 (5): 567-74. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Menge, C. et al. (2004) Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection.
J Med Microbiol. 53: 573-9.
View The Latest Product References
-
Rhodes, S.G. et al. (1999) Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.
J Gen Virol. 80 : 1673-9. -
Piper, E.K. et al. (2009) Immunological profiles of Bos taurus and Bos indicus cattle infested with the cattle tick, Rhipicephalus (Boophilus) microplus.
Clin Vaccine Immunol. 16: 1074-86. -
Woolums, A.R. et al. (2013) Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves.
Am J Vet Res. 74: 343-54. -
Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
Immunology. 96 (3): 396-403. -
Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
Pol J Vet Sci. 15: 11-20. -
Menge, C. et al. (2003) Verotoxin 1 from Escherichia coli affects Gb3/CD77+ bovine lymphocytes independent of interleukin-2, tumor necrosis factor-alpha, and interferon-alpha.
Exp Biol Med (Maywood). 228: 377-86. -
Menge, C. et al. (1999) Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro.
Infect Immun. 67: 2209-17. -
Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75. -
Maślanska, T. and Jaroszewski, J.J. (2013) In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25-CD4+ cells.
J Vet Sci. 14 (2): 125-34. -
Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10. -
Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
Dev Comp Immunol. 47 (2): 254-63. -
Kang, H.J. et al. (2016) Effects of Ambient Temperature on Growth Performance, Blood Metabolites, and Immune Cell Populations in Korean Cattle Steers.
Asian-Australas J Anim Sci. 29 (3): 436-43. -
Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
Vet Res. 49 (1): 28. -
Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
Vet Immunol Immunopathol. 202: 74-84. -
Brodzki, P. et al. (2020) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. 91 (1): e13306. -
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388. -
de Araújo, F.F. et al. (2019) Distinct immune response profile during Rhipicephalus (Boophilus) microplus. infestations of guzerat dairy herd according to the maternal lineage ancestry (mitochondrial DNA).
Vet Parasitol. 273: 36-44. -
Colombatti, M.O. et al. (2021) Evaluation of a virulent strain of Mycobacterium avium subsp. paratuberculosis used as a heat-killed vaccine.
Vaccine. 39 (51): 7401-12. -
Tucker, N. et al. (2023) Bovine blood and milk T-cell subsets in distinct states of activation and differentiation during subclinical Staphylococcus aureus mastitis.
J Reprod Immunol. 156: 103826. -
Blanco, F.C. et al. (2021) Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells.
Front Immunol. 12: 674643. -
Seemann, L. et al. (2024) Dietary L-carnitine supplementation modifies blood parameters of mid-lactating dairy cows during standardized lipopolysaccharide-induced inflammation.
Front Immunol. 15: 1390137.
- Synonyms
- IL-2R Alpha Chain
- UniProt
- P12342
- Entrez Gene
- IL2RA
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
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