Mouse anti Bovine CD18, clone BAQ30A
, recognizes bovine CD18 also known as ITGB2 or integrin beta-2. CD18 is a receptor for ICAM1, ICAM2, ICAM3, and ICAM4. It recognizes the G-P-R motif in the fibrinogen alpha chain and is involved in leukocyte adhesion as well as transmigration of T cells and neutrophils.
Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica
binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage (Shanthalingam et al. 2016
). Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome (Kehrli et al. 1992
) with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections (Jeyaseelan et al. 2000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD18 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul