CD18 antibody | BAQ30A
Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage (Shanthalingam et al. 2016). Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome (Kehrli et al. 1992) with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections (Jeyaseelan et al. 2000).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep Bison Water Buffalo Cat Dog Alpaca Llama Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Preparation
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Cells from multiple species
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P32592
- Entrez Gene
- ITGB2
- GO Terms
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007275 multicellular organismal development
- Regulatory
- For research purposes only
Applications of CD18 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Functional Assays |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Bovine CD11c | MCA6084 | F IC | 0.1 mg |
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Mouse anti Bovine CD14 | MCA6085 | F | 0.1 mg |
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Positive Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG1:RPE | STAR132PE | F | 0.1 mg |
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Product Specific References
References for CD18 antibody
-
Howard C.J. & Naessens J (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Saalmüller A. et al. (2005) Summary of the animal homologue section of HLDA8.
Cell Immunol. 236 (1-2): 51-8.
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