CD34 antibody | AV138
(Image courtesy of The Roslin Institute).
(Image courtesy of the Roslin Institute).
Mouse anti Chicken CD34
- Product Type
- Monoclonal Antibody
- Clone
- AV138
- Isotype
- IgG1
- Specificity
- CD34
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD34 antibody, clone AV138 recognizes chicken CD34, a cell surface antigen expressed by, endothelial progenitor cells, endothelial cells and haematopoietic stem cells. Mouse anti Chicken CD34 antibody, clone AV138 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD34 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from Bio-Rad. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken CD34/huIg fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunocytochemistry 1 | |
||
| Western Blotting | |
- 1It has been demonstrated that this antibody can be used to detect chicken CD34 expressed in COS7 cells using an AEC substrate detection system.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in indirect ELISA applications.
- Western Blotting
- Clone AV138 detects a band of approximately 70 - 80kDa on COS cell supernatant transfected with chicken CD34 (extracellular)/V5HIS6 tagged protein under reducing conditions.
Further Reading
-
Cairns, D.M. et al. (2012) Interplay of Nkx3.2, Sox9 and Pax3 regulates chondrogenic differentiation of muscle progenitor cells.
PLoS One. 7: e39642.
- UniProt
- E1BUT3
MCA5936GA
158184
163826
180702
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD34 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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