Talin Antibody | TA205

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Human peripheral blood platelets stained with Mouse anti Human Talin (MCA725G)

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  • Talin Antibody | TA205 thumbnail image 1
  • Mouse anti Human Talin
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  • Product Type
    Monoclonal Antibody
  • Clone
    TA205
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA725GC, F *, IF, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA725G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a 225 kDa cytoskeletal protein. It may be used to stain adhesion plaques in fixed and permeabilised fibroblasts. TA205 recognizes an epitope within residues 139-433.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/251/100
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Most tissues
    • Immunofluorescence
    • Western Blotting
      Clone TA205 has also been reported to work in Western blotting.
    • Instructions For Use

    Additional Talin Antibody Formats

    Formats Clone Applications Sizes available
    Talin Antibody : Purified TA205 C, F *, IF, IP, WB 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Most tissues
              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

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