Talin Antibody | TA205

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Talin Antibody | TA205 gallery image 1

Human peripheral blood platelets stained with Mouse anti Human Talin (MCA725G)

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Talin Antibody | TA205 gallery image 2

Published customer image:
Mouse anti Human talin antibody, clone TA205 (MCA725G)used to isolate and recognize talin from A375 human melanoma cell line lysates by immunoprecipitation and western blotting respectively
Image caption:
c-Abl kinase directly associates with talin head domain and regulates β3 integrin ligation concomitantly after PDGF stimulation.
(A) Overnight serum-starved A375 cells were either untreated or treated with 20 ng/ml PDGF for the indicated times. Thereafter, the cells were lysed, and the lysates were immunoblotted with indicated antibodies. In inhibition experiments, cells were preincubated with STI571 for 10 min, and the inhibitor remained in the medium throughout the assay period. (B) Serum-starved A375 cells were stimulated as above. The lysates were immunoprecipitated with anti-talin antibody and immunoblotted with indicated antibodies. (C) Serum-starved A375 cells were treated as above. The lysates were incubated with GST or GST fusion proteins, and the bound proteins were separated by SDS-PAGE and immunoblotted with indicated antibodies. (D) GST or GST fusion proteins were incubated with Ni2+ Sepharose coated with His-talin2-head. Bound proteins were separated by SDS-PAGE and immunoblotted with anti-GST antibody. The immunoblotting bands were quantified by densitometry, and data were normalized with respect to the controls. Data are representative of three independent experiments.

From: Zhang C, Yang C, Wang R, Jiao Y, Ampah KK, Wang X, et al. (2013)
c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
PLoS ONE 8(6): e66108.

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  • Mouse anti Human Talin
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  • Product Type
    Monoclonal Antibody
  • Clone
    TA205
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA725GC, F*, IF, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA725G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/251/100
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Most tissues
    • Immunofluorescence
    • Western Blotting
      Clone TA205 has also been reported to work in Western blotting.
    • Instructions For Use

    Additional Talin Antibody Formats

    Formats Clone Applications Sizes available
    Talin Antibody : Purified TA205 C, F*, IF, IP, WB 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Most tissues
              • Application NameReference Images
                Immunofluorescence
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              References

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