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Ryanodine Receptor Antibody | RYR.1 (G-1)

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  • Mouse anti Human Ryanodine Receptor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    RYR.1 (G-1)
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1818C, E, F, IB, IF*datasheet pdfdatasheet pdf50 µg
    OBT1818
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 recognizes the human ryanodine receptor 1, also known as Skeletal muscle calcium release channel or skeletal muscle ryanodine receptor. Ryanodine receptor 1 is a is a large 5038 amino acid ~500 kDa multi pass sarcoplamic reticulum associated transmembrane protein containing three B30.2/SPRY domains and five MIR domains. Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 recognizes an epitope within the C-terminal cytoplasmic domain of the ryanodine receptor (UniProt: P21817).

      Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 reacts with ryanodine receptor isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues). Defects in the ryanodine receptor 1 gene are associated with malignant hyperthermia (Gillard et al. 1992), central core disease of muscle (Quane et al. 1993) and certain congential myopathies (Monnier et al. 2000).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1000
      Flow Cytometry1/500
      Immunoblotting1/100
      Immunohistology - Frozen1/100
      Immunofluorescence(1)1/100
      (1)
      Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ryanodine Receptor Antibody Formats

    Formats Clone Applications Sizes available
    Ryanodine Receptor Antibody : Purified RYR.1 (G-1) C, E, F, IB, IF* 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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