Protein Gene Product 9.5 Antibody | 13C4

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Protein Gene Product 9.5 Antibody | 13C4 gallery image 1

Rat brain probed with Mouse anti Human Protein Gene Product 9.5 (7863-2004)

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Protein Gene Product 9.5 Antibody | 13C4 gallery image 2

Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 used for the detection of PGP9.5 immunoreactive nerve fibers in porcine gastric mucosa by immunofluorescence.
Image caption:
Tissue sampling and cell measurements. (a) Picture presenting the interior surface of the "experimental animal" stomach, exposed by cutting along the greater curvature of the stomach. Ulcers are indicated by red arrows. (b) Microphotograph showing Haematoxylin and Eosin staining of the gastric ulcer margin. The gastric mucosa shows a defect (red ellipse with the inscription “Ulcer” and arrows) that extends into the deep muscular layers. (c) Diagram showing the method of tissue sampling. The tracer injection site is indicated by the violet arrows. The red vertical stripes symbolize the subsequent 0.5 cm thick transverse sections taken in specific intervals (section I—1.5 cm; section II—3.5cm; section III—5.5 cm; section IV—7.5cm) starting from the pyloric sphincter (section 0—blue vertical stripe). Subsequently, each of these sections (I, II, III, IV) was cut into 20 µm thick cryostat consecutive microscopic slices for future processing. The red circle symbolizes the localization of ulcer induced in experimental animals. (d, e, f) Photomicrographs showing: (d) bundles of PGP-immunoreactive fibers (arrowheads) and perikarya (arrows). Double arrow points to the perikaryon which was fast blue (FB) positive [the perimeter (l) and area (A) of the cell body are included]; (e) fast blue (FB) positive cell body (double arrow) with lines indicating its dimensions; (f) the image formed by merging both channels (green: PGP 9.5 and blue: FB).

From: Zalecki M (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)—Neuronal Tracing Studies.
PLoS ONE 10(5): e0126958.

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  • Mouse anti Human Protein Gene Product 9.5
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  • Product Type
    Monoclonal Antibody
  • Clone
    13C4
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7863-2004E, P *, WBdatasheet pdfdatasheet pdf0.2 ml
    7863-2004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human protein gene product 9.5 antibody, clone 13C4 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.

      This product stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Also stains neuroendocrine cells in human adult gut (unlike 31A3).

      Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/1000
      Immunohistology - Paraffin(1)1/200
      (1)
      Antigen is stable in formalin fixed paraffin embedded sections, however we recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding. Can be used without acid/ethanol fixation if the sections are subjected to microwave treatment in citrate buffer by standard methods.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Protein Gene Product 9.5 Antibody Formats

    Formats Clone Applications Sizes available
    Protein Gene Product 9.5 Antibody : Purified 13C4 E, P *, WB 0.2 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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              • Application NameReference Images
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              References

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