MCP-1 Antibody | 2.2-4A4-1A11

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MCP-1 Antibody | 2.2-4A4-1A11 gallery image 1

Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MCA2468) following antigen retrieval with sodium citrate buffer (pH6.0)

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Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MCA2468) following antigen retrieval with sodium citrate buffer (pH6.0)

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MCP-1 Antibody | 2.2-4A4-1A11 gallery image 3

Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 (MCA2486) as a capture reagent and biotinylated Rabbit anti Human MCP-1 (AHP784B) as a detection reagent with recombinant human MCP-1 (PHP061) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are included undiluted

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  • Mouse anti Human MCP-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2.2-4A4-1A11
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2486E, P *, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA2486
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes human C-C motif chemokine 2, also known as Monocyte chemoattractant protein 1 (MCP-1), Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2 or CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. MCP-1 is a 99 amino acid, including a 23 aa signal peptide ~13kDa chemotactic protein, a chemoattractant for monocytes and basophils but not neutrophils or eosinophils (Weber et al.1996).

      MCP-1 is produced by a number of cell types, including monocytes, macrophages, microglia, fibroblasts and epithelial cells (Cushing et al. 1990). MCP-1 influences T-cell immunity polarizing responses towards the Th2 phenotype (Karpus and Kennedy 1997). Variations in the CCL2 gene affects susceptibility to Mycobacteriun tuberculosis infection (Flores-Villanueva et al. 2005).

      Mouse anti Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes both native and recombinant MCP-1 in conjunction with Rabbit anti Human MCP-1 polyclonal antibody in a sensitive sandwich ELISA assay.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1.02.0ug/ml
      Western Blotting0.20.4ug/ml
      Immunohistology - Paraffin(1)10ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP784B as the detection reagent and PHP061 as the standard.
    • Immunohistology
    • Histology Positive Control Tissue
      Human Breast Invasive Ductal Carcinoma
    • Immunofluorescence
    • Western Blotting
      This product may be used under either reducing or non-reducing conditions together with PHP061 as the positive control.
    • Instructions For Use

    Additional MCP-1 Antibody Formats

    Formats Clone Applications Sizes available
    MCP-1 Antibody : Purified 2.2-4A4-1A11 E, P *, WB 0.5 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human Breast Invasive Ductal Carcinoma

              References

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