Cytokeratin 14 Antibody | LL002

//www.bio-rad-antibodies.com/human-cytokeratin-14-antibody-ll002-mca890.html?RenderPageSpecific=1
Cytokeratin 14 Antibody | LL002 gallery image 1

Immunoperoxidase staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human cytokeratin 14 (MCA890) folllowing heat mediated antigen retrieval using citrate buffer, pH6.0

Enlarge
Cytokeratin 14 Antibody | LL002 gallery image 2

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 (MCA890) followed by HRP polymer detection and DAB substrate development (low power) following heat mediated antigen retrieval using citrate buffer at pH6.2

Enlarge
Cytokeratin 14 Antibody | LL002 gallery image 3

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 (MCA890) followed by HRP polymer detection and DAB substrate development (high power) following heat mediated antigen retrieval using citrate buffer at pH6.2

Enlarge
Cytokeratin 14 Antibody | LL002 gallery image 4

Published customer image:
Mouse anti Human cytokeratin 14 antibody, clone LL002 used for the detection of CK14 expressing cells in a canine inflammatory mammary cancer pellet section by immunohistochemistry.
Image caption:
Immunohistochemistry of IPC-366 pellet, paraffin sections. A) Pancytokeratin (AE1/AE3) (10X); intense immunolabeling and numerous positive cells. B) CK14; intense immunolabeling and numerous positive cells (10X). C) Vimentin; moderate immunolabeling and numerous positive cells (10X). D) p63; negative (20X). E) a-smooth muscle actin, negative cells (10X). F) Estrogen receptor; negative cells (20X). G) Progesterone receptor; negative cells (20X). H) HER-2; negative cells (20X). I) E-Cadherin; intense membranous immunolabeling in numerous cells (20X). J) COX-2; most of the cells are moderately positive; some cells are intensely positive (10X). K) COX-2; some cells strongly positive showing features of malignancy (binucleated cell, arrow) (40X). L) Ki-67 proliferation marker; many nuclei are positive (10X).

From: Caceres S, Peña L, de Andres PJ, Illera MJ, Lopez MS, et al. (2015) Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366. PLoS ONE 10(3): e0122277.

Enlarge
  • Cytokeratin 14 Antibody | LL002 thumbnail image 1
  • Cytokeratin 14 Antibody | LL002 thumbnail image 2
  • Cytokeratin 14 Antibody | LL002 thumbnail image 3
  • Cytokeratin 14 Antibody | LL002 thumbnail image 4
  • Mouse anti Human Cytokeratin 14
  • Mouse anti Human Cytokeratin 14
  • Mouse anti Human Cytokeratin 14
  • Mouse anti Human Cytokeratin 14:FITC
  • Mouse anti Human Cytokeratin 14
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LL002
  • Isotype
    IgG3
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA890FF *, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA890F
    MCA890C, F *, IF, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA890
    MCA890HTC, IF, P *datasheet pdfdatasheet pdf1 ml
    MCA890HT
    MCA890TC, F *, IF, P *datasheet pdfdatasheet pdf20 µg
    MCA890T
    MCA890HC, IF, P *datasheet pdfdatasheet pdf5 ml
    MCA890H
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14.

      Mouse anti cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011)
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Elephantyes
      Dogyes
      Pigyes
      Lionyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.5%Bovine Serum Albumin
      0.09%Sodium Azide
      0.5%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cytosol
      structural constituent of cytoskeleton
      epidermis development
      keratin filament
      mitochondrion
      protein binding
      nucleus
      hemidesmosome assembly
      intermediate filament bundle assembly
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)1/100
      Immunohistology - Paraffin(2)1/200
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)Neat
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
      Enzyme digestion is not recommended.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)Neat
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
      Enzyme digestion is not recommended.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)1/100
      Immunohistology - Paraffin(2)1/200
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
      * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
      Enzyme digestion is not recommended.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Skin
    • Histology Positive Control Tissue
      Skin
    • Histology Positive Control Tissue
      Skin
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Skin
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Cytokeratin 14 Antibody Formats

    Formats Clone Applications Sizes available
    Cytokeratin 14 Antibody : Purified LL002 C, F *, IF, P *, WB 0.2 mg | 20 µg
    Cytokeratin 14 Antibody : Ready To Use LL002 C, IF, P * 1 ml | 5 ml
    Cytokeratin 14 Antibody : FITC LL002 F *, IF 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG3:FITCHCA039F0.1 mgF
      HCA039F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG3:HRPHCA039P0.1 mgE
      HCA039P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG3:RPEHCA039PE100 TestsF
      HCA039PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG3:FITCHCA039F0.1 mgF
      HCA039F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG3:HRPHCA039P0.1 mgE
      HCA039P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG3:RPEHCA039PE100 TestsF
      HCA039PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG3:FITCHCA039F0.1 mgF
      HCA039F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG3:HRPHCA039P0.1 mgE
      HCA039P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG3:RPEHCA039PE100 TestsF
      HCA039PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG3:FITCHCA039F0.1 mgF
      HCA039F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG3:HRPHCA039P0.1 mgE
      HCA039P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG3:RPEHCA039PE100 TestsF
      HCA039PE

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Leucoperm™BUF0950 TestsF
          BUF09
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Skin
              Skin
              Skin
              Skin

              References

              Write your review

              You may also be interested in...