Cytochrome B245 Antibody | CS9

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Cytochrome B245 Antibody | CS9 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain (MCA4686). Permeabilised with Leucoperm

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Cytochrome B245 Antibody | CS9 gallery image 2

Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor® ?488 (MCA4686A488). Permeabilised with Leucoperm

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Cytochrome B245 Antibody | CS9 gallery image 3

Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor® ?647 (MCA4686A647). Permeabilised with Leucoperm

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Cytochrome B245 Antibody | CS9 gallery image 4

Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:FITC (MCA4686F). Permeabilised with Leucoperm

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  • Cytochrome B245 Antibody | CS9 thumbnail image 1
  • Cytochrome B245 Antibody | CS9 thumbnail image 2
  • Cytochrome B245 Antibody | CS9 thumbnail image 3
  • Cytochrome B245 Antibody | CS9 thumbnail image 4
  • Mouse anti Human Cytochrome B245 Light Chain
  • Mouse anti Human Cytochrome B245 Light Chain
  • Mouse anti Human Cytochrome B245 Light Chain:Alexa Fluor® 488
  • Mouse anti Human Cytochrome B245 Light Chain:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CS9
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4686GAC, E, F *, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4686GA
    MCA4686C, E, F *, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA4686
    MCA4686A488F *datasheet pdfdatasheet pdf100 Tests/1ml
    MCA4686A488
    MCA4686A647F *datasheet pdfdatasheet pdf100 Tests/1ml
    MCA4686A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.

      The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.

      The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor 488 - liquid
      Purified IgG conjugated to Alexa Fluor 647 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Cytochrome B245 solubilised in octyl-beta-gluycopyranoside.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 0.05mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the 3PU1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      protein heterodimerization activity
      stored secretory granule
      electron carrier activity
      SH3 domain binding
      smooth muscle hypertrophy
      cytochrome complex assembly
      electron transport chain
      transport
      heme binding
      inflammatory response
      hydrogen peroxide biosynthetic process
      respiratory burst
      NADPH oxidase complex
      innate immune response
      superoxide anion generation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/101/100
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)1/101/100
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA4686GA detects a band of approximately 19kDa in neutrophil membrane extracts
    • Western Blotting
      MCA4686 detects a band of approximately 19kDa in neutrophil membrane extracts
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Cytochrome B245 Antibody Formats

    Formats Clone Applications Sizes available
    Cytochrome B245 Antibody : Alexa Fluor® 647 CS9 F * 100 Tests/1ml
    Cytochrome B245 Antibody : Purified CS9 C, E, F *, IP, WB 0.2 mg | 0.1 mg
    Cytochrome B245 Antibody : Alexa Fluor® 488 CS9 F * 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA928A488100 Tests/1mlF
        MCA928A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647

        Useful Reagents

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          Human SeroblockBUF070B200 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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              References

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