CD9 Antibody | MM2/57

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CD9 Antibody | MM2/57 gallery image 1

Staining of acetone fixed frozen human tonsil with Mouse anti Human CD9 (MCA469GA)

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CD9 Antibody | MM2/57 gallery image 2

Staining of human peripheral blood monocytes with Mouse anti Human CD9:Alexa Fluor® 647 (MCA469A647)

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CD9 Antibody | MM2/57 gallery image 3

Figure A. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse anti Human CD9 (MCA469PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A)

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CD9 Antibody | MM2/57 gallery image 4

Staining of human peripheral blood platelets with Mouse anti Human CD9:Biotin (MCA469B)

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CD9 Antibody | MM2/57 gallery image 5

Staining of human peripheral blood platelets with Mouse anti Human CD9:FITC (MCA469F)

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CD9 Antibody | MM2/57 gallery image 6

Staining of human peripheral blood platelets with Mouse anti Human CD9:RPE (MCA469PE)

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  • CD9 Antibody | MM2/57 thumbnail image 1
  • CD9 Antibody | MM2/57 thumbnail image 2
  • CD9 Antibody | MM2/57 thumbnail image 3
  • CD9 Antibody | MM2/57 thumbnail image 4
  • CD9 Antibody | MM2/57 thumbnail image 5
  • CD9 Antibody | MM2/57 thumbnail image 6
  • Mouse anti Human CD9:FITC
  • Mouse anti Human CD9:Biotin
  • Mouse anti Human CD9
  • Mouse anti Human CD9
  • Mouse anti Human CD9:FITC
  • Mouse anti Human CD9:Biotin
  • Mouse anti Human CD9:RPE
  • Mouse anti Human CD9:Alexa Fluor® 647
  • Mouse anti Human CD9:RPE
  • Mouse anti Human CD9
  • Mouse anti Human CD9:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MM2/57
  • Isotype
    IgG2b
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA469GAC, F, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA469GA
    MCA469FFdatasheet pdfdatasheet pdf0.1 mg
    MCA469F
    MCA469BFdatasheet pdfdatasheet pdf0.1 mg
    MCA469B
    MCA469GC, F, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA469G
    MCA469PEFdatasheet pdfdatasheet pdf100 Tests
    MCA469PE
    MCA469A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA469A647
    MCA469FTFdatasheet pdfdatasheet pdf25 µg
    MCA469FT
    MCA469BTFdatasheet pdfdatasheet pdf25 µg
    MCA469BT
    MCA469GTC, F, WBdatasheet pdfdatasheet pdf25 µg
    MCA469GT
    MCA469PETFdatasheet pdfdatasheet pdf25 Tests
    MCA469PET
    MCA469A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA469A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9. CD9 is a 228 amino acid multi pass membrane glycoprotein belonging to the tetraspanin family with a molecular weight of ~24 kDa expressed by platelets, monocytes, some lymphocytes and endothelial cells.

      Mouse anti Human CD9 antibody, clone MM2/57 recognizes a conserved epitope on CD9 present on a wide range of mammalian species.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Catyes
      Rhesus Monkeyyes
      Bovineyes
      Dogyes
      Rabbityes
      Horseyes
      Pigyes
      MustelidExpected from Sequence
      Minkyes
      Llamayes
      Ferretyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid.
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid.
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1ml distilled water.
      Pack Size: 100 TestsReconstitute with 1ml distilled water.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Human platelet membranes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      platelet activation
      protein binding
      cell adhesion
      fusion of sperm to egg plasma membrane
      platelet alpha granule membrane
      integral to plasma membrane
      platelet degranulation
      paranodal junction assembly
      cellular component movement
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen1/5001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen1/5001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/2
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/2
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen1/5001/1000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD9 Antibody Formats

    Formats Clone Applications Sizes available
    CD9 Antibody : Alexa Fluor® 647 MM2/57 F 25 Tests/0.25ml | 100 Tests/1ml
    CD9 Antibody : Purified MM2/57 C, F, WB 0.1 mg | 25 µg | 0.2 mg
    CD9 Antibody : FITC MM2/57 F 0.1 mg | 25 µg
    CD9 Antibody : Biotin MM2/57 F 25 µg | 0.1 mg
    CD9 Antibody : RPE MM2/57 F 25 Tests | 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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