CD79a Antibody | HM57

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CD79a Antibody | HM57 gallery image 1

Staining of paraffin embedded human tonsil with Mouse anti Human CD79a (MCA2538)

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  • CD79a Antibody | HM57 thumbnail image 1
  • Mouse anti Human CD79a:RPE
  • Mouse anti Human CD79a:Alexa Fluor® 647
  • Mouse anti Human CD79a
  • Mouse anti Human CD79a:Alexa Fluor® 647
  • Mouse anti Human CD79a:RPE
  • Mouse anti Human CD79a
  • Mouse anti Human CD79a
  • Mouse anti Human CD79a
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  • Product Type
    Monoclonal Antibody
  • Clone
    HM57
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2538GAC, F *, P *datasheet pdfdatasheet pdf0.1 mg
    MCA2538GA
    MCA2538C, F *, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2538
    MCA2538HC, F *, P *, WBdatasheet pdfdatasheet pdf1 ml
    MCA2538H
    MCA2538PEF *datasheet pdfdatasheet pdf100 Tests
    MCA2538PE
    MCA2538A647F *datasheet pdfdatasheet pdf100 Tests/1ml
    MCA2538A647
    MCA2538TC, F *, P *, WBdatasheet pdfdatasheet pdf25 µg
    MCA2538T
    MCA2538PETF *datasheet pdfdatasheet pdf25 Tests
    MCA2538PET
    MCA2538A647TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2538A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells.

      The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Dogyes
      Rabbityes
      Horseyes
      Pigyes
      Monkeyyes
      Red deeryes
      Ferretyes
      Goatyes
      Ratyes
      Bovineyes
      Chickenyes
      Guinea Pigyes
      Fallow deeryes
      American Bisonyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor®647 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor®647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1ml distilled water
      Pack Size: 25 TestsReconstitute with 0.25ml distilled water
      Pack Size: 100 TestsReconstitute with 1ml distilled water
      Pack Size: 25 TestsReconstitute with 0.25ml distilled water
    • Preparation
      Pack Size: 100 TestsPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 TestsPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 1 ml, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 100 TestsPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 TestsPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 1 ml, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 1 ml, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 1 ml, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 1 ml
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 25 µg, 0.1 mg, 0.2 mg
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
      Pack Size: 1 ml
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 25 µg, 0.1 mg, 0.2 mg
      0.09%Sodium Azide (NaN3)
      Pack Size: 1 ml
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 25 µg, 0.1 mg, 0.2 mg
      0.09%Sodium Azide (NaN3)
      Pack Size: 1 ml
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 25 µg, 0.1 mg, 0.2 mg
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05mg/ml
      Pack Size: 1 mlIgG concentration 0.1mg/ml
      Pack Size: 25 µg, 0.1 mg, 0.2 mgIgG concentration 1.0mg/ml
      IgG concentration 0.05mg/ml
      Pack Size: 1 mlIgG concentration 0.1mg/ml
      Pack Size: 25 µg, 0.1 mg, 0.2 mgIgG concentration 1.0mg/ml
      Pack Size: 1 mlIgG concentration 0.1mg/ml
      Pack Size: 25 µg, 0.1 mg, 0.2 mgIgG concentration 1.0mg/ml
      Pack Size: 1 mlIgG concentration 0.1mg/ml
      Pack Size: 25 µg, 0.1 mg, 0.2 mgIgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Pack Size: 100 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      B cell receptor complex
      external side of plasma membrane
      integral to membrane
      B cell receptor signaling pathway
      B cell proliferation
      multivesicular body
      B cell differentiation
      membrane raft
      transmembrane receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/51/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)
      1/10Pack Size: 0.2 mg, 1 ml, 25 µg
      1/50Pack Size: 0.1 mg
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/25Pack Size: 1 ml
      Immunohistology - Paraffin(2)1/1001/500
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/51/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)
      1/10Pack Size: 0.2 mg, 1 ml, 25 µg
      1/50Pack Size: 0.1 mg
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/25Pack Size: 1 ml
      Immunohistology - Paraffin(2)1/1001/500
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)
      1/10Pack Size: 0.2 mg, 1 ml, 25 µg
      1/50Pack Size: 0.1 mg
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/25Pack Size: 1 ml
      Immunohistology - Paraffin(2)1/1001/500
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)
      1/10Pack Size: 0.2 mg, 1 ml, 25 µg
      1/50Pack Size: 0.1 mg
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/25Pack Size: 1 ml
      Immunohistology - Paraffin(2)1/1001/500
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD79a Antibody Formats

    Formats Clone Applications Sizes available
    CD79a Antibody : Purified HM57 C, F *, P *, WB 1 ml | 0.1 mg | 0.2 mg | 25 µg
    CD79a Antibody : Alexa Fluor® 647 HM57 F * 25 Tests/0.25ml | 100 Tests/1ml
    CD79a Antibody : RPE HM57 F * 100 Tests | 25 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              Human tonsil
              Human tonsil
              Human tonsil

              References

              Further Reading

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