CD74 Antibody | Bu45

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CD74 Antibody | Bu45 gallery image 1

Figure A. RPE-A750 tandem conjugated Mouse anti Human CD19 (MCA1940P750) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-A750 tandem conjugated Mouse anti Human CD19 (MCA1940P750) and FITC conjugated Mouse anti Human CD74 (MCA2502F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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  • CD74 Antibody | Bu45 thumbnail image 1
  • Mouse anti Human CD74:FITC
  • Mouse anti Human CD74
  • Mouse anti Human CD74:FITC
  • Mouse anti Human CD74
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Bu45
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2502FF *datasheet pdfdatasheet pdf0.1 mg
    MCA2502F
    MCA2502C *, F *, FN*, IF, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2502
    MCA2502FTF *datasheet pdfdatasheet pdf25 µg
    MCA2502FT
    MCA2502TC *, F *, IF, IPdatasheet pdfdatasheet pdf25 µg
    MCA2502T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kD isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes.

      CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
    • Immunogen
      B lymphoblastoid cell line HFB1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/5
      (1)
      Membrane permeabilisation may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Flow Cytometry(1)1/251/50
      Functional Assays(2)
      Immunohistology - Frozen(3)
      (1)
      Membrane permeabilisation may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
      (2)
      "This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose. "
      (3)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/5
      (1)
      Membrane permeabilisation may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Flow Cytometry(1)1/251/50
      Functional Assays(2)
      Immunohistology - Frozen(3)
      (1)
      Membrane permeabilisation may enhance CD74 staining. Bio-Rad recommend the use of LeucopermTM (product code BUF09) for this purpose.
      (2)
      "This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose. "
      (3)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD74 Antibody Formats

    Formats Clone Applications Sizes available
    CD74 Antibody : FITC Bu45 F * 25 µg | 0.1 mg
    CD74 Antibody : Purified Bu45 C *, F *, FN*, IF, IP 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              References

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