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CD68 antibody | 514H12

Product Code Applications Pack Size List Price Your Price Qty
MCA1815T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF P * 0.1 ml loader
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loader
MCA1815
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF P * 1 ml loader
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loader

Mouse anti Human CD68 antibody, clone 514H12 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Target Species
Human
Product Form
Tissue culture supernatant - liquid
Preservative Stabilisers
MCA1815T: <0.1% sodium azide (NaN3)
MCA1815: 0.09% sodium azide (NaN3)
Immunogen
Fusion protein corresponding to external domain of human CD68.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/40 1/80
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. N.B. The epitope recognised by clone 514H12 is sensitive to peroxide. It is therefore recommended that any peroxidase-blocking steps be performed after incubation with this antibody.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

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References for CD68 antibody

  1. da Costa, C.E. et al. (2005) Presence of osteoclast-like multinucleated giant cells in the bone and nonostotic lesions of Langerhans cell histiocytosis.
    J Exp Med. 201 (5): 687-93.
  2. Rodriguez-Agudo, D. et al. (2006) Localization of StarD5 cholesterol binding protein.
    J Lipid Res. 47: 1168-75.
  3. Paulmyer-Lacroix, O. et al. (2006) Expression of adrenomedullin in adipose tissue of lean and obese women.
    Eur J Endocrinol. 155: 177-85.
  4. Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
    J Transl Med. 4: 30.
  5. Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
    Int Immunol. 19: 1271-9.
  6. Achard, V. et al. (2007) Renin receptor expression in human adipose tissue.
    Am J Physiol Regul Integr Comp Physiol. 292: R274-82.
  7. Hever, A. et al. (2007) Human endometriosis is associated with plasma cells and overexpression of B lymphocyte stimulator.
    Proc Natl Acad Sci U S A. 104: 12451-6.
  8. Silaghi, A. et al. (2007) Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status.
    Am J Physiol Endocrinol Metab. 293: E1443-50.
  9. View The Latest Product References
  10. Kaibara, N. et al. (2008) Comparative histopathological analysis between tenosynovitis and joint synovitis in rheumatoid arthritis.
    Histopathology. 52: 856-64.
  11. Moskovszky, L. et al. (2009) Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis.
    Genes Chromosomes Cancer. 48: 468-79.
  12. Muthana, M. et al. (2011) Use of macrophages to target therapeutic adenovirus to human prostate tumors.
    Cancer Res. 71 (5): 1805-15.
  13. de Vos van Steenwijk PJ et al. (2013) Tumor-infiltrating CD14-positive myeloid cells and CD8-positive T-cells prolong survival in patients with cervical carcinoma.
    Int J Cancer. 133 (12): 2884-94.
  14. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
    PLoS One. 8 (5): e65121.
  15. Toh, M.L. et al. (2014) Bone- and cartilage-protective effects of a monoclonal antibody against colony-stimulating factor 1 receptor in experimental arthritis.
    Arthritis Rheumatol. 66 (11): 2989-3000.
  16. Schirmer, L. et al. (2014) Differential loss of KIR4.1 immunoreactivity in multiple sclerosis lesions.
    Ann Neurol. 75 (6): 810-28.
  17. Bobosha, K. et al. (2014) T-cell regulation in lepromatous leprosy.
    PLoS Negl Trop Dis. 8 (4): e2773.
  18. Quispel, W.T. et al. (2016) Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis.
    Oncoimmunology. 5 (8): e1164364.
  19. Schrevel, M. et al. (2017) Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer.
    Int J Oncol. 50 (6): 1947-54.
  20. van Poppel, P.C.M. et al. (2018) Inflammation in the subcutaneous adipose tissue does not attenuate endothelial function in subjects with diabetes mellitus and subjects with dyslipidaemia and hypertension: A cross-sectional study.
    Endocrinol Diabetes Metab. 1 (3): e00020.
  21. Yuan, A. et al. (2020) Histologic analysis of medication-related osteonecrosis of the jaw compared with antiresorptive-exposed bone and other infectious, inflammatory, and necrotic jaw diseases.
    Oral Surg Oral Med Oral Pathol Oral Radiol. 129 (2): 133-40.
  22. Durán, D. et al. (2020) Histological and Immunohistochemical Study of Wounds in Sheep Skin in Maggot Therapy by Using Protophormia terraenovae. (Diptera: Calliphoridae.) Larvae.
    J Med Entomol. 57 (2): 369-76.
  23. Nowak-Sliwinska, P. et al. (2022) Proinflammatory activity of VEGF-targeted treatment through reversal of tumor endothelial cell anergy.
    Angiogenesis. Dec 02 [Epub ahead of print].
  24. Cossins, B.C. et al. (2023) Sex-specific association between adipose tissue inflammation and vascular and metabolic complications of obesity.
    J Clin Endocrinol Metab. dgad193.
  25. Perga, S. et al. (2021) Overexpression of the ubiquitin-editing enzyme A20 in the brain lesions of Multiple Sclerosis patients: moving from systemic to central nervous system inflammation.
    Brain Pathol. 31 (2): 283-96.

Immunofluorescence

Immunohistology - Paraffin

Synonyms
Macrosialin
RRID
AB_322866
UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane

MCA1815T

151806 155577 157856 161606 162025 162475

MCA1815

149438 153548 157780 161622 167814

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