CD62L Antibody | FMC46

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CD62L Antibody | FMC46 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human CD62L:FITC (MCA1076F)

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  • CD62L Antibody | FMC46 thumbnail image 1
  • Mouse anti Human CD62L
  • Mouse anti Human CD62L:RPE
  • Mouse anti Human CD62L:RPE
  • Mouse anti Human CD62L
  • Mouse anti Human CD62L
  • Mouse anti Human CD62L:FITC
  • Mouse anti Human CD62L:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    FMC46
  • Isotype
    IgG2b
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1076GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1076GA
    MCA1076FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1076F
    MCA1076GC, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA1076G
    MCA1076PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1076PE
    MCA1076GTC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA1076GT
    MCA1076FTFdatasheet pdfdatasheet pdf25 µg
    MCA1076FT
    MCA1076PETFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1076PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD62L antibody, clone FMC46 recognizes human CD62L, also known a L-selectin, a 74-95kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62-P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes.

      CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains.

      The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      Dogyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid.
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised.
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid.
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water.
      Pack Size: 100 TestsReconstitute with 1 ml distilled water.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      PHA stimulated lymphoblasts.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      leukocyte migration
      glycosphingolipid binding
      cell adhesion
      integral to plasma membrane
      blood coagulation
      heparin binding
      regulation of immune response
      sugar binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD62L Antibody Formats

    Formats Clone Applications Sizes available
    CD62L Antibody : FITC FMC46 F 25 µg | 0.1 mg
    CD62L Antibody : Purified FMC46 C, F, IP 0.2 mg | 0.1 mg | 25 µg
    CD62L Antibody : RPE FMC46 F 25 Tests/0.25ml | 100 Tests
    • Copyright © 2016 Bio-Rad

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              References

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