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CD59 antibody | MEM-43/5

Product Code Applications Pack Size List Price Your Price Qty
MCA1927
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F WB 0.2 mg loader
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loader

Mouse anti Human CD59 antibody, clone MEM-43/5 reacts with a PI-linked glycoprotein of ~18-20 kDa found on all types of leucocytes including platelets. The antigen is expressed on many haemopoietic and non-haemopoietic cells. Mouse anti Human CD59 antibody, clone MEM-43/5 recognizes an epitope distinct to that bound by Mouse anti Human CD59 antibody, cloneMEM-43 (MCA1054).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
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Description Mouse IgG2b Negative Control

Antibody Characterization Reference

  1. Stefanová I et al. (1989) Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.
    Mol Immunol. 26 (2): 153-61.

References for CD59 antibody

  1. Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
    Folia Biol (Praha). 34 (1): 23-34.
  2. Stefanova, I., et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens.
    Ed. Knapp, W. et al. Oxford University Press pp 678-97.
  3. Tandon, N. et al. (1994) Expression and function of multiple regulators of complement activation in autoimmune thyroid disease.
    Immunology. 81 (4): 643-7.
  4. Vanderplasschen, A. et al. (1998) Extracellular enveloped vaccinia virus is resistant to complement because of incorporation of host complement control proteins into its envelope.
    Proc Natl Acad Sci U S A. 95 (13): 7544-9.
  5. Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
    J Clin Invest. 116: 249-60.
  6. Takemoto, M. et al. (2007) Human herpesvirus 7 infection increases the expression levels of CD46 and CD59 in target cells.
    J Gen Virol. 88: 1415-22.
  7. Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
    Cell Div. 2: 20.

Flow Cytometry

Immunohistology - Frozen

Western Blotting

Synonyms
HRF
Protectin
RRID
AB_323387
UniProt
P13987
Entrez Gene
CD59
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0007166 cell surface receptor linked signaling pathway
GO:0031362 anchored to external side of plasma membrane

MCA1927

0615 161571 161721

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