CD3 Antibody | MEM-57

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CD3 Antibody | MEM-57 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:FITC (MCA2184F)

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CD3 Antibody | MEM-57 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE (MCA2184PE)

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CD3 Antibody | MEM-57 gallery image 3

Figure A. FITC conjugated mouse anti human CD3 (MCA2184A488F) and Alexa®647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD3 (MCA2184F) and Alexa®647 conjugated mouse anti human CD4 (MCA1267A647). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

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  • CD3 Antibody | MEM-57 thumbnail image 1
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  • Mouse anti Human CD3
  • Mouse anti Human CD3:RPE
  • Mouse anti Human CD3:FITC
  • Mouse anti Human CD3:FITC
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    CD3 antibody, clone MEM-57, recognizes an extracellular epitope of the human CD3 complex which is expressed on the surface of circulating human peripheral T cells, thymocytes, Purkinji cells and neurons present in the cerebellar cortex.
    • Product Type
      Monoclonal Antibody
    • Clone
      MEM-57
    • Isotype
      IgG2a
    3 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA2184FFdatasheet pdfdatasheet pdf0.1 mg
      MCA2184F
      MCA2184F, IPdatasheet pdfdatasheet pdf0.2 mg
      MCA2184
      MCA2184PEFdatasheet pdfdatasheet pdf100 Tests
      MCA2184PE
      MCA2184FTFdatasheet pdfdatasheet pdf25 µg
      MCA2184FT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either δ-ε or γ-ε subunit complexes (Dave et al. 1997). Mouse anti Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3ε subunit (Transy et al. 1989) as it will recognize murine 3D054.8 hybridoma cells tranfected with cDNA to express the human CDε subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells.

        The TCR complex consists of TCR heterodimers, either α/β or γ/δ expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, γ, δ, ε and ζ responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006 Dave et al. 1997).

        Mouse anti Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batista et al. 2004 Brdicková et al. 2003). Clone MEM-57 immunoprecipitates proteins of ~22 kDa and more weakly ~28 kDa. from peripheral blood T cell lysates.
      • Intended Use
      • Target Species
        Human
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Chimpanzeeyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      • Reconstitution
        Reconstitute with 1 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A.
        Purified IgG prepared by affinity chromatography on Protein A.
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Purity
        >95% by SDS-PAGE
      • Approx. Protein Concentrations
        IgG concentration 1 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        G-protein coupled receptor protein signaling pathway
        receptor signaling protein activity
        protein kinase binding
        cytoplasm
        transmembrane receptor activity
        SH3 domain binding
        establishment or maintenance of cell polarity
        positive thymic T cell selection
        integral to plasma membrane
        regulation of apoptosis
        T cell receptor signaling pathway
        protein heterodimerization activity
        protein complex assembly
        T cell costimulation
        external side of plasma membrane
        T cell receptor binding
        T cell activation
        integral to membrane
        receptor signaling complex scaffold activity
        signal complex assembly
        protein transport
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/51/2
        Immunoprecipitation
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/10

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : FITC MEM-57 F 0.1 mg | 25 µg
      CD3 Antibody : Purified MEM-57 F, IP 0.2 mg
      CD3 Antibody : RPE MEM-57 F 100 Tests
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
        STAR117A
        Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
        STAR87A
        Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
        STAR117D488GA
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
        STAR117D549GA
        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
        STAR117D680GA
        Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
        STAR117D800GA
        Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
        STAR8D800GA
        Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
        HCA037F
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
        STAR70
        Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
        STAR120F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
        HCA037P
        Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
        STAR117P
        Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
        STAR77
        Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
        STAR120P
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
        STAR87P
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A
        Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
        STAR76

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG2a Negative ControlMCA929100 TestsF
          MCA929
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG2a Negative Control:RPEMCA929PE100 TestsF
          MCA929PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
          MCA929F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
          MCA929F

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human SeroblockBUF070B200 TestF
            BUF070B
            Human SeroblockBUF070A50 TestF
            BUF070A

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Flow Cytometry

                References

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