CD239 Antibody | BRIC221

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CD239 Antibody | BRIC221 gallery image 1

Published customer image:
Mouse anti Human CD239 antibody, clone BRIC221 used to look for blocking of Lu-Fc to endogenous α5 laminin in kidney sections.
Image caption:
Lu/B-CAM binding assay on tissue sections.
(A) Binding of Lu-Fc to laminin α5 in adult mouse kidney sections. Sections were incubated with Lu-Fc (upper panel) or Fc (lower panel) at room temperature for 1 hour. Endogenous laminin α5 and bound Lu-Fc were detected with anti-laminin α5 domain LEb/L4b and anti-human IgG, as indicated. Merged pictures showed that laminin α5 and Lu-Fc co-localized at basement membranes. Arrows indicate glomeruli, laminin α5-rich structures. Bar, 100 μm. (B) Binding of Lu-Fc to laminin α5 in adult mouse skeletal muscle sections. Endogenous laminin α2 (upper panel) and α5 (lower panel) were detected with antisera. Bound Lu-Fc was detected with an antibody against human IgG as described above. Lu-Fc only bound to the basement membranes containing α5, primarily neuromuscular junctions. Arrowheads indicate neuromuscular junctions. Bar, 50 μm. (C) Inhibitory effects of anti-Lu/B-CAM antibodies on Lu-Fc binding to laminin α5. After preincubation with anti-Lu/B-CAM antibodies, Lu-Fc was used in the binding assay on tissue sections. Antibody 87207 inhibited the binding of Lu-Fc to endogenous laminin α5.

From: Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M (2011)
An Antibody to the Lutheran Glycoprotein (Lu) Recognizing the LU4 Blood Type Variant Inhibits Cell Adhesion to Laminin α5.
PLoS ONE 6(8): e23329.

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CD239 Antibody | BRIC221 gallery image 2

Published customer image:
Mouse anti Human CD239 antibody, clone BRIC221 epitope mapping by adsorbtion with lutheran deletion and chimeric mutant proteins as measured by ELISA
Image caption:
Identifying the IgSF domains recognized by anti-Lu/B-CAM antibodies.
(A) Diagram of the deletion and chimeric mutant proteins designed to narrow the epitopes of anti-Lu/B-CAM antibodies. For chimeric mutant proteins, the D1 or D1–D2 domains of Lu-Fc were replaced with the analogous domains of melanoma cell adhesion molecule (Mel-CAM). The deletion and chimeric mutant proteins were fused with an IgG Fc domain tag. (B) The mutant proteins purified from conditioned media of HEK293 transfectants were subjected to SDS-PAGE on a 7.5% gel under reducing conditions. Protein was stained with Coomassie Brilliant Blue. Molecular mass standards are indicated. (C) ELISA using the indicated anti-Lu/B-CAM antibodies absorbed with the various recombinant proteins. Wells were coated with 3 μg/ml of Sol-Lu. The mutant proteins were mixed with the diluted antibodies at 1 μg/ml. Each bar represents the mean of triplicate assays. Error bars indicate standard deviation.

From: Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M (2011)
An Antibody to the Lutheran Glycoprotein (Lu) Recognizing the LU4 Blood Type Variant Inhibits Cell Adhesion to Laminin α5.
PLoS ONE 6(8): e23329.

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  • Mouse anti Human CD239
  • Mouse anti Human CD239
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    BRIC221
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1982C, E, F, IF, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA1982
    MCA1982TC, E, F, IF, WB*datasheet pdfdatasheet pdf25 µg
    MCA1982T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD239 antibody, clone BRIC221 recognizes human CD239, also known as Lutheran antigen or basal cell aghesion molecule.CD239 is a 597 amino acid, ~85 kDa single pass type I membrane glycoprotein. Clone BRIC221 recognizes a monomorphic determinant expresses on both the 85 and 78 kDa Lutheran (Lu) glycoforms (El Nemer et al. 1998). BRIC 221 recognizes an epitope in the fourth extracellular domain of Lu glycoprotein (Parsons et al. 1997). Lutheran glycoprotein is a member of the immunoglobulin superfamily and was designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Human erythrocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Pack Size: 0.2 mgTRIS buffered glycine
      Pack Size: 25 µgPhosphate buffered saline
      Pack Size: 0.2 mgTRIS buffered glycine
      Pack Size: 25 µgPhosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cell-matrix adhesion
      external side of plasma membrane
      laminin receptor activity
      laminin binding
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting(1)
      (1)
      Non-reducing conditions required
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting(1)
      (1)
      Non-reducing conditions required

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD239 Antibody Formats

    Formats Clone Applications Sizes available
    CD239 Antibody : Purified BRIC221 C, E, F, IF, WB* 25 µg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

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              References

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