Alpha Fetoprotein Antibody | 2I9/2

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Alpha Fetoprotein Antibody | 2I9/2 gallery image 1

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein (4520-5306) as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (0200-0562) as a detection reagent with human alpha fetoprotein (Bio-Rad Catalog 13752600) as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

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Alpha Fetoprotein Antibody | 2I9/2 gallery image 2

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein (4520-5306) as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (MCA5862) as a detection reagent with human alpha fetoprotein (Bio-Rad Catalog 13752600) as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

Enlarge
Alpha Fetoprotein Antibody | 2I9/2 gallery image 3

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein (MCA5862) as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (4520-5306) as a detection reagent with human alpha fetoprotein (Bio-Rad Catalog 13752600) as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

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  • Mouse anti Human Alpha Fetoprotein
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  • Product Type
    Monoclonal Antibody
  • Clone
    2I9/2
  • Isotype
    IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4520-5306Edatasheet pdfdatasheet pdf1 mg
    4520-5306
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 4520-5306 recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults.

      Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down’s syndrome.

      4520-5306 does not cross-react with human albumin and other human serum proteins.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Ig fraction - liquid
    • Reconstitution
    • Preparation
      Ig fraction prepared by chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Human fetal cord serum
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Alpha Fetoprotein Antibody Formats

    Formats Clone Applications Sizes available
    Alpha Fetoprotein Antibody : Purified 2I9/2 E 1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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