Actin Beta Antibody | 4C2

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Actin Beta Antibody | 4C2 gallery image 1

Western blot analysis of Hela whole cell lysate probed with Mouse anti Human actin beta antibody (MCA5775GA) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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Sandwich ELISA analysis of human actin beta binding using Human anti Human actin beta antibody, clone 12141 (HCA147) as a capture reagent and biotinylated Mouse anti Human actin, clone 4C2 (MCA5775GA) as a detection reagent with purified human actin beta as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Asthmatic sufferer serum samples (orange and blue) and SLE serum sample (green) are shown. Beta actin was undetectable in normal serum samples

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Published customer image:
Mouse anti Human actin antibody, clone 4C2 used for the demonstration of actin expression in epithelial cells by immunofluorescence.
Image caption:
Normal and transformed IAR epithelial cells. (A) Single IAR-2 epithelial cells are discoid, they form islands in sparse culture and a confluent monolayer in dense culture. Scale bar 10 µm. (B) E-cadherin and N-cadherin expression in normal and transformed IAR cells. (C) IAR-2 cells were stained for E-cadherin (green) and actin (red). Top row (1.25 µm above the substrate) shows AJs organized as adhesion belts encircling each cell and co-localizing with circumferential actin bundles in the apical parts of cells. Bottom row (substrate level) shows parts of adhesion belts and irregular distributions of actin. Scale bar 10 μm. (D) Transformed IAR-6-1 cells were stained for E-cadherin (green) and actin (red). In these cells, radial AJs were associated with thin actin bundles. Scale bar 10 μm.

From: Rubtsova SN, Zhitnyak IY, Gloushankova NA (2015) A Novel Role of E-Cadherin-Based Adherens Junctions in Neoplastic Cell Dissemination.
PLoS ONE 10(7): e0133578.

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Published customer image:
Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in normal and neoplasic cells by immunofluorescence and western blotting.
Image caption:
Expression of cytoplasmic actins in normal and transformed cells (A-C); expression of cytoplasmic actins and HaCaT cellular characteristics (D-G) A. Immunofluorescent staining for β-actin (green) and γ-actin (red) of representative pairs of NSCLC and Colon Cancer samples with matching normal tissue samples. Scale bars represent 50μm. Graphs represent staining intensity of biopsies (Mean ± SD). B. WB analysis of HaCaT cells with exogenous expression of active N-RasD13. Graphs represent relative actins expression (Mean ± SD). C. Immunofluorescent staining for β-actin (green) and γ-actin (red) of N-RasD13 transformed HaCaT cells. Scale bars represent 10 μm. D. WB analysis of HaCaT cells with exogenous expression of β- or γ-actins and corresponding shRNAs. shC is sh to GFP. Graphs represent relative actins expression (Mean ± SD). E. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in cells with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). F. Immunofluorescent staining of HaCaT cells with altered β- or γ-actins expression. Scale bars represent 10 μm. G. HaCaT cells with silenced or exogenously expressed non-muscle cytoplasmic actins that crossed the matrigel-coated membranes (left). Graphs represent mean ± SD. HaCaT proliferation dynamics with exogenous expression or silenced of β- or γ-actins (right). Error bars are SD.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

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Published customer image:
Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.
Image caption:
β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

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Published customer image:
Mouse anti Human Actin beta; antibody, clone 4C2 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.
Image caption:
β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

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Published customer image:
Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin and ERK1/2 are mutually regulated A. WB analysis of A549 cells with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative pERK1/2 expression (Mean ± SD). B. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple) or pERK1/2 (red) immunofluorescent staining. Scale bars represent 10 μm. C. pERK1/2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. pERK1/2/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD). E. LSM of A549 cells treated with EGF or UO126 with β-actin (green) or γ-actin (red) immunofluorescent staining. Scale bars represent 10 μm. F. WB analysis of A549 cells treated with EGF or UO126. Graphs represent relative pERK1/2 or β-/γ-actin levels (Mean ± SD).

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

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Published customer image:
Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with p34-Arc and WAVE2 and regulates them A. p34-Arc (red) or γ-actin (green) immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. B. γ-Actin (red) or WAVE2 (green) immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. C. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). D. p34-Arc immunoprecipitation analysis of A549 cells with down-regulated β- or &gamma-actins. E. WAVE2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. F. LSM of A549 cells with down-regulated β-actin with p34-Arc (green, upper panel), WAVE2 (green, lower panel), β-actin (red) or γ-actin (purple) immunofluorescent staining. Scale bars represent 2 μm. Asterisk marks the cell with silenced β-actin.

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

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Actin Beta Antibody | 4C2 gallery image 9

Published customer image:
Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with cofilin1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple), cofilin1 (red) or DAPI (blue) immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). C. Cofilin1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. Cofilin1/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD).

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

Enlarge
Actin Beta Antibody | 4C2 gallery image 10

Published customer image:
Mouse anti Human Actin beta; antibody, clone 4C2 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.
Image caption:
γ-actin co-localizes with PP1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin (green), γ-actin (purple), PP1α (red) or DAPI (blue) immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative PP1&alpha levels (Mean ± SD). C. PP1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. PP1α/γ-actin (upper panel) and PP1α/β-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. E. Comparative quantification of PP1α-β-/γ-actin PLA dots. Graph represents relative fluorescence intensity (Mean ± SD).

From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.
Oncotarget. 2015 Jun 10;6(16):14556-71.

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  • Mouse anti Human Actin Beta
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4C2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5775GAC, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5775GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human actin beta, clone 4C2, recognises cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein.

      Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhove et al. 1978).

      Clone 4C2 is highly specific for cytoplasmic actin β and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodelling (Miazza et al. 2011)
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rabbityes
      Pigyes
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to KLH.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen1/501/100
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Actin Beta Antibody Formats

    Formats Clone Applications Sizes available
    Actin Beta Antibody : Purified 4C2 C, IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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