Starbright Violet 790 Dye
StarBright Violet 790 (SBV790) Dye is the latest addition to the StarBright Dye range of fluorescent nanoparticles specifically designed for flow cytometry.
Excitable by the violet 405 nm laser and emitting at 782 nm, it can be detected using the 750LP filter on the ZE5 Cell Analyzer or similar filters on other instruments.
Fig. 1. Excitation and emission spectra for SBV790. The dye is excited maximally by the violet laser (dotted line) with minimal excitation by other lasers. The emission profile is narrow to reduce spillover into neighboring filters.
StarBright Violet 790 Dye is also compatible with spectral flow cytometry and has a unique profile, allowing incorporation into new and existing flow cytometry panels giving greater flexibility and increasing multiplex capabilities. In fact, the unique profile of SBV790 allows you to combine this dye with Brilliant Violet 785 (BV785) in a panel. The full spectral profile is shown below (Figure 2).
Fig. 2. Dye signature of SBV790. The full emission spectrum of StarBright Violet 790 Dye showing the emission at all wavelengths. Data was collected on the Cytek Aurora Flow Cytometry System using SpectroFlo Software.
SBV790 Dye has been designed to be bright with narrow excitation and emission, making it a great choice for inclusion into multicolor panels. It is up to 2x brighter than dyes with similar excitation and emission profiles (Figure 3), allowing detection of rare and low antigen density populations, removal of lineage positive cells, and great separation from negative cells in all multicolor panels.
Fig. 3. Brightness comparison and spectral properties. Human peripheral blood was stained with CD4SBV790 (MCA1267SBV790) (red) or CD4BV785 (blue). Both antibodies were titrated prior to use to determine the optimal concentration. SBV790 stain index was 2x that observed for BV785.
No special staining buffer is required, SBV790 Dye has been tested in most common staining buffers and special staining buffers for polymer dyes, with no drop in performance, so it can be used in new or existing panels, with no change to your experimental protocol (Figure 4). StarBright Violet 790 Dye is very stable, when stored at 4oC, and is not susceptible to photobleaching. The staining does not deteriorate with fixation.
This dye has also high lot-to-lot reproducibility, so you can expect the same high-quality staining regardless of the lot purchased.
StarBright Violet 790 Dye is compatible with all common fluorophores such as organic fluorophores, protein fluorophores, polymer dyes, and other StarBright Dyes in multicolor panels, without the requirement for a special staining protocol or special staining buffer. Examples of staining with other dyes, including StarBright Dyes, can be seen in Figure 5. If you are using multiple dyes that require a special buffer, we recommend using that buffer, as StarBright Dye performance will not be affected.
Fig. 5. Examples of SBV790 Dye staining. Human or mouse peripheral blood was stained with A, CD8SBV440 (MCA1226SBV440) and CD4SBV790 (MCA1267SBV790); B, CD4BV421 and CD4SBV790 (MCA1267SBV790); C, CD3A488 (MCA463A488) and CD28SBV790 (MCA709SBV790); and D, CD11PE (MCA74PE) and CD117SBV790 (MCA1365SBV790). All staining was performed at 4oC in PBS containing 1% BSA after blocking with 10% human serum. Cells were analyzed on the ZE5 Cell Analyzer.
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For more information on how the StarBright Dye range can fit into your flow cytometry experiments, please take a look at our StarBright Dyes page.
For more information about fluorophores and immunophenotyping, refer to our flow cytometry resources.
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