CD20 antibody | 2H7
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Mouse anti Human CD20:StarBright Violet 515
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.
The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.
The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 515 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 515 401 516
- For research purposes only
- 12 months from date of despatch
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
DO NOT FREEZE.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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- Select combined or multi-laser view to visualize the spectra
References for CD20 antibody
Chan, H.T. et al. (2003) CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts.
Cancer Res. 63: 5480-9.
Cragg, M.S. et al. (2003) Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts.
Blood. 101: 1045-52.
Jaramillo, M.C. et al. (2009) Increased manganese superoxide dismutase expression or treatment with manganese porphyrin potentiates dexamethasone-induced apoptosis in lymphoma cells.
Cancer Res. 69: 5450-7.
Teeling, J.L. et al. (2006) The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20.
J Immunol. 177 (1): 362-71.
Polyak, M.J. et al. (2002) Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20
monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
Greig, B. et al. (2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.
Cytometry B Clin Cytom. 86: 135-8.
van den Akker, E. et al. (2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.
Haematologica. 95: 1594-8.
Jaramillo, M.C. et al. (2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.
Free Radic Biol Med. 83: 89-100.
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