CD8 antibody | LT8

Mouse anti Human CD8:StarBright Violet 710

Product Type
Monoclonal Antibody
Clone
LT8
Isotype
IgG1
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA1226SBV710
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Mouse anti Human CD8 antibody, clone LT8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells.

The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Marmoset
Chimpanzee
Cynomolgus monkey
Red-bellied Tamarin
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 710 - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Normal human peripheral blood lymphocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 710 401 713
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD8 antibody

  1. Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
    J Immunol. 167 (8): 4593-9.
  2. Manninen, A. & Saksela, K. (2002) HIV-1 Nef interacts with inositol trisphosphate receptor to activate calcium signaling in T cells.
    J Exp Med. 195 (8): 1023-32.
  3. Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
    Adv Immunol. 44: 265-311.
  4. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
    J Histochem Cytochem. 57: 1159-67.
  5. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12: 2.
  6. Nelson, M. et al. (2010) Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus).
    J Med Microbiol. 59: 1107-13.
  7. Gibbings, D.J. et al. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.
    BMC Immunol. 8: 12.
  8. Junker, A. et al. (2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.
    Brain. 130: 2789-99.
  9. View The Latest Product References
  10. Held, K. et al. (2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.
    J Virol. 85 (19): 9680-5.
  11. Hood SP et al. (2014) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).
    Virus Res. 179: 93-101.
  12. Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.
    J Immunol Res. 2014: 913632.
  13. Manivannan, K. et al. (2016) CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.
    PLoS Pathog. 12 (4): e1005560.
  14. Gross, C.C. et al. (2016) Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation.
    Proc Natl Acad Sci U S A. 113 (21): E2973-82.
  15. Dunham, J. et al. (2016) Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.
    J Neuroimmune Pharmacol. 11 (1): 73-83.
  16. Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
    PLoS One. 12 (7): e0180088.
  17. Philippens, I.H. et al. (2017) Acceleration of Amyloidosis by Inflammation in the Amyloid-Beta Marmoset Monkey Model of Alzheimer's Disease.
    J Alzheimers Dis. 55 (1): 101-113.

Flow Cytometry

Immunofluorescence

UniProt
P01732
P10966
Entrez Gene
CD8A
CD8B
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
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