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CD14 antibody | TÜK4

Mouse anti Human CD14:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
TÜK4
Isotype
IgG2a
Specificity
CD14

Product Code Applications Pack Size List Price Your Price Qty
MCA1568A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Mouse anti Human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989), a soluble form of CD14 also exists (Bazil et al. 1986).

CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.

Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003). Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Goat
Cat
Rabbit
Mink
Bovine
Pig
Sheep
Cynomolgus monkey
Llama
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 488 MCA929A488 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG2a Negative Control:Alexa Fluor® 488

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD14 antibody

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  2. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
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  3. Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
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  4. Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.
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  5. Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
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    Am J Respir Crit Care Med. 165: 1602-9.
  7. Yang, H. et al. (2003) Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion.
    Immunol Lett. 88 (1): 27-30.
  8. Schenk, M. et al. (2005) Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.
    J Immunol. 174 (1): 517-24.
  9. View The Latest Product References
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    Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95.
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  20. Schaut, R.G. et al. (2015) Bovine viral diarrhea virus type 2 in vivo infection modulates TLR4 responsiveness in differentiated myeloid cells which is associated with decreased MyD88 expression.
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  21. Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
    Vet Comp Oncol. 14 (4): 409-16.
  22. Gibson, A.J. et al. (2016) Differential macrophage function in Brown Swiss and Holstein Friesian cattle.
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  23. Krueger, L.A. et al. (2016) Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves.
    J Dairy Sci. 99 (11): 9040-50.
  24. Lund, H. et al. (2016) Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
    Front Immunol. 7: 322.
  25. Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
    J Diabetes Res. 2016: 3486727.
  26. Pomeroy, B. et al. (2017) Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
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    J Feline Med Surg. 20 (6): 494-501.
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  29. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
    Vet Immunol Immunopathol. 202: 74-84.
  30. Lessard, M. et al. (2018) Piglet weight gain during the first two weeks of lactation influences the immune system development.
    Vet Immunol Immunopathol. 206: 25-34.
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  33. Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
    Comp Immunol Microbiol Infect Dis. 68: 101388.
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    Am J Pathol. 190 (6): 1188-210.
  35. Sipka, A.S. et al. (2020) The effect of ex vivo. lipopolysaccharide stimulation and nutrient availability on transition cow innate immune cell AKT/mTOR pathway responsiveness.
    J Dairy Sci. 103 (2): 1956-1968.
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    Viruses. 12 (3): 291.
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    Animal. 14 (4): 780-9.
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    Vet Comp Oncol. 19 (3): 567-77.
  42. Jaensch, S.M. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia.
    Aust Vet J. 100 (11): 527-32.
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    J Immunother Cancer. 10(5):e004007.
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    Biomater Res. 26 (1): 79.
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    Curr Issues Mol Biol. 44 (12): 5827-38.
  47. Ashwood, P. (2022) Preliminary Evidence of Differentially Induced Immune Responses by Microparticle-adsorbed LPS in Patients with Crohn's Disease.
    J Cell Immunol. 4 (6): 211-218.
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    J Feline Med Surg. 24 (2): 77-90.
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  51. Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
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Further Reading

  1. Bazil, V. et al. (1986) Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.
    Eur J Immunol. 16:1583-9.
  2. Simmons, D. L. et al. (1989) Monocyte antigen CD14 is a phospholipid anchored membrane protein.
    Blood. 73:284-9.
  3. Pugin, J. et al. (1994) CD14 is a pattern recognition receptor.
    Immunity.1:509-16.
  4. Dziarski, R. et al. (1998) Binding of bacterial peptidoglycan to CD14.
    J Biol Chem. 273:8680-90.
  5. Jersmann, H.P. (2005) Time to abandon dogma: CD14 is expressed by non-myeloid lineage cells.
    Immunol Cell Biol. 83:462-7.
  6. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Immuno-electron Microscopy

UniProt
P08571
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0001530 lipopolysaccharide binding
GO:0001847 opsonin receptor activity
GO:0006915 apoptosis
GO:0006909 phagocytosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0031225 anchored to membrane
GO:0016019 peptidoglycan receptor activity
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0070891 lipoteichoic acid binding
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid

MCA1568A488

155396

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