CD19 antibody | LT19
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors.
(A) Immunofluorescent staining of human melanoma and normal human skin tissue sections; anti-CD19 (red; B cell marker) and anti-p-STAT3 (green). Scale bars, 20 μm.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors.
(B) B cells in primary tumor sites impact overall tumor STAT3 activity. Confocal microscopic images showing primary melanoma tumor tissue staining of B cells and p-STAT3 (left), with quantification of CD19 and p-STAT3 positive cells (right). Scale bars, 20 μm. Total ten microscopic fields (10 X) were examined for each tumor section; n = 2.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 express pro-angiogenic genes and accumulate around microvessels in human tumors.
(A) B cells are important for expression of pro-angiogenic genes within human prostate tumor tissues. The density of B cells around tumor vasculature in prostate tumor tissue was determined by immunofluorescent staining using anti-CD19 and anti-CD31 antibodies (top); scale bars, 20 μm. Real-time RT-PCR measuring RNA expression levels of pro-angiogenic genes in the consecutive human prostate tumor tissue sections (bottom). The relative amount of mRNA is normalized to 18S and compared to RNA levels in tumor tissues with high p-STAT3, which is designated as 1; means ± SD, n = 2.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 express pro-angiogenic genes and accumulate around microvessels in human tumors.
(B) Immunofluorescent staining of human prostate tumor tissue sections showing B cells and microvessels with CD31+ endothelial cells (green). Scale bars, 100μm in the original (top) and 20μm in the enlarged (bottom).
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors and their density in the tumor tissues reflects overall STAT3 activity.
(A) Immunofluorescent staining of many other types of human cancers; anti-CD19 (red; B cell marker) and anti-p-STAT3 (green). Scale bars, 50μm in the original and 10μm in the enlarged.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors and their density in the tumor tissues reflects overall STAT3 activity.
(B) Immunofluorescent staining showing CD19- and p-STAT3 (green) and nuclei (DAPI, blue). Scale bars, 10μm.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunohistochemistry on formalin fixed, paraffin embedded tissue sections..
Image caption:
B cells accumulate around microvessels in human prostate tumors.
IHC images showing the accumulation of CD19-positive B cells in human prostate tumor tissues; H&E staining of the consecutive tissue sections.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunohistochemistry on formalin fixed, paraffin embedded tissue sections..
Image caption:
B cells with activated STAT3 express pro-angiogenic genes and accumulate around microvessels in human tumors.
(C) B cells around the microvessels display persistently activated STAT3. IHC showing B cells and p-STAT3-positive cells in the same area of human prostate tumor tissues; scale bars, 200 μm. H&E staining of the consecutive tissue sections. Microvessel-like structures are marked by red dots; scale bars, 200μm in the original and 50μm in the enlarged.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19:RPE-Alexa Fluor® 750
- Product Type
- Monoclonal Antibody
- Clone
- LT19
- Isotype
- IgG1
- Specificity
- CD19
Antibody Quality and Performance Guarantee
| Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989). CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradbury et al. 1992), CD9, CD81 and CD82 (Horváth et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE-Alexa Fluor®750 488nm laser 496 779 RPE-Alexa Fluor®750 561nm laser 546 779 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
Neat |
- 1Tandem conjugates of RPE - Alexa Fluor 750 display fluorescence properties that are virtually identical to those of RPE - Cy7 tandem conjugates.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or cells or 100μl whole blood
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD19 antibody
-
Hughes, G.J. et al. (2007) Virus immunocapture provides evidence of CD8 lymphocyte-derived HIV-1 in vivo.
AIDS. 21: 1507-13. -
Allen, J.S. et al. (2009) Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture.
Diabetes. 58: 138-45. -
McIntosh, K. et al. (2006) The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Stem Cells. 24: 1246-53. -
Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
Int Immunol. 18 (7): 1171-8. -
Villarroel Dorrego, M. et al. (2006) Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules.
Br J Dermatol. 154: 231-8. -
Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
Blood. 118: 348-57. -
Lacal, P.M. et al. (2013) Glucocorticoid-induced tumor necrosis factor receptor family-related ligand triggering upregulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 and promotes leukocyte adhesion.
J Pharmacol Exp Ther. 347: 164-72. -
Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
Blood. 118: 348-57.
View The Latest Product References
-
Girbl, T. et al. (2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.
Cancer Res. 73: 561-70. -
Hertzberg, L. et al. (2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.
Blood. 115: 1006-17. -
Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
Diab Vasc Dis Res. 8: 221-8. -
Dorvignit, D. et al. (2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.
MAbs. 4 (4): 488-96. -
Karlsen, M. et al. (2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.
Scand J Immunol. 82 (6): 523-31. -
Clark, L.E. et al. (2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.
Nat Commun. 9 (1): 1884. -
Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
Nat Commun. 10 (1): 893. -
Yang, C. et al. (2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.
PLoS One. 8 (5): e64159.
- RRID
- AB_808397
- UniProt
- P15391
- Entrez Gene
- CD19
- GO Terms
- GO:0005515 protein binding
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0006968 cellular defense response
MCA1940P750
151774
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