IgG2 Antibody | CA4F1

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IgG2 Antibody | CA4F1 gallery image 1

Purified Dog IgG detected with Mouse anti Dog IgG2 (MCA2328GA) followed by Goat anti Mouse IgG1:HRP (STAR132P)

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  • Product Type
    Monoclonal Antibody
  • Clone
    CA4F1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2328GAE, F, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2328GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Dog IgG2 antibody, clone CA4F1 recognizes the canine IgG2 heavy chain. ELISA tests have shown that this clone does not react with canine IgG1, IgA or IgM (Arce et al. 2002). Mouse anti Dog IgG2, clone CA3H1 does not react with the Fab portion of canine IgG or recognize heat, acid and periodate treated canine IgG.

      Competition ELISA demonstrated that anti dog IgG2 clones CA3H1 and CA4F1 were able to completely inhibit the binding of each other to IgG2 indicating that both clones recognize the same or closely related epitopes.
    • Intended Use
    • Target Species
      Dog
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      Rabbityes
      Sheepyes
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Canine IgG
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/15,0001/150,000
      Flow Cytometry
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG2 Antibody Formats

    Formats Clone Applications Sizes available
    IgG2 Antibody : Purified CA4F1 E, F, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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