IgG2 Antibody | IL-A73

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IgG2 Antibody | IL-A73 gallery image 1

Bovine IgG2 detected in bovine serum with Mouse anti Bovine IgG2 (MCA2441GA) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B)

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  • Product Type
    Monoclonal Antibody
  • Clone
    IL-A73
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2441GAE, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2441GA
    MCA2441PEdatasheet pdfdatasheet pdf0.25 mg
    MCA2441P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine IgG2, clone IL-A73, is a monoclonal antibody specific for bovine IgG2 and does not recognise other bovine immunoglobulin classes.

      While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races.

      Clone IL-A73 is reported to immunoprecipitate a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 see Campbell, J.D.M. et al. for details.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Purified bovine IgG2
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG2 Antibody Formats

    Formats Clone Applications Sizes available
    IgG2 Antibody : Purified IL-A73 E, F, IP 0.1 mg
    IgG2 Antibody : HRP IL-A73 E 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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