CD4 Antibody | CC8

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CD4 Antibody | CC8 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:Alexa Fluor® 647 (MCA1653A647)

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CD4 Antibody | CC8 gallery image 2

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:FITC (MCA1653F)

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CD4 Antibody | CC8 gallery image 3

Published customer image:
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the evaluation of CD4 expression on proliferating bovine peripheral blood mononuclear cells by flow cytometry.
Image caption:
Determination of phenotypes of proliferating PBMC by FACS analysis. Determination of phenotypes (CD4+, CD8+ and WC1+) of proliferating PBMC by CFDA SE staining after re-stimulating with respective vaccine antigen and fluorescence-activated cell sorter (FACS) analysis. PBMC were separated from heparinised blood of vaccinated cattle originated from full dose vaccine group on 4 weeks after homologous FMDV challenge. Two naïve cattle without FMDV infection and two A Malaysia 97 FMDV infected cattle (4 weeks post-infection) without prior vaccination were included as control group. PBMCs from naïve animals were re-stimulated with SAT2 Eritrea vaccine antigen. FACS plots were exemplified only for one vaccinated challenged animal from SAT2 Eritrea animal experiment. Similar FACS plots were obtained for the PBMC originated from A Malaysia 97 and SAT2 Eritrea vaccinated animals. Error bars represent standard error of the mean.

From: Oh Y, Fleming L, Statham B, Hamblin P, Barnett P, Paton DJ, et al. (2012) Interferon-γ Induced by In Vitro Re-Stimulation of CD4+ T-Cells Correlates with In Vivo FMD Vaccine Induced Protection of Cattle against Disease and Persistent Infection.
PLoS ONE 7(9): e44365.

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CD4 Antibody | CC8 gallery image 4

Published customer image:
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of CD4 expressing lymphocytes in preipheral blood by flow cytometry.
Image caption:
Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN-γ within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN-γ and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.

From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.

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CD4 Antibody | CC8 gallery image 5

Published customer image:
Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653) used for the identification of CD4 expressing lymphocytes in preipheral blood by flow cytometry.
Image caption:
Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN-γ, IL-2 and TNF-α determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN-γ, IL-2 or TNF-α following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN-γ+ and IFN-γ- CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle.

From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.

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CD4 Antibody | CC8 gallery image 6

Published customer image:
APC conjugated Mouse anti Bovine CD4 antibody, clone CC8 used for the identification of CD4 expressing T-cells in bovine peripheral blood by flow cytoometry together with RPE conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837PE) used to detect cytotoxic T-cells in the same samples.
Image caption:
Insert image caption

From: Sun F, Cao Y, Cai C, Li S, Yu C, Yao J (2016)
Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
PLoS ONE 11(8): e0160659.

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  • Mouse anti Bovine CD4:RPE
  • Mouse anti Bovine CD4:FITC
  • Mouse anti Bovine CD4
  • Mouse anti Bovine CD4:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC8
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1653FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1653F
    MCA1653GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1653GA
    MCA1653PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1653PE
    MCA1653A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1653A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskra et al. 1991).
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Bovine lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunized mouse were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Purified CC8 C, F, IP 0.1 mg
    CD4 Antibody : FITC CC8 F 0.1 mg
    CD4 Antibody : RPE CC8 F 100 Tests
    CD4 Antibody : Alexa Fluor® 647 CC8 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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