CD34 Antibody | AV138

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CD34 Antibody | AV138 gallery image 1

Direct ELISA analysis of chicken His tagged CD34 transfected COS-7 cells detected with Mouse anti Chicken CD34 (MCA5936GA) [red], anti HIS tag [blue] and COS cells transfected with CD14extracellular\V5HIS plasmids used as a negative control [green]
(Image courtesy of The Roslin Institute)

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CD34 Antibody | AV138 gallery image 2

Immunocytochemical staining of chicken CD34 transfected COS-7 cells (A) with Mouse anti Chicken CD34 (MCA5936GA) detected using an AEC detection system. Mock transfected cells (B)
(Image courtesy of the Roslin Institute)

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  • CD34 Antibody | AV138 thumbnail image 1
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  • Mouse anti Chicken CD34
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AV138
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5936GAE, IC*, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5936GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Clone AV138 is specific for chicken CD34, a cell surface antigen expressed by, endothelial progenitor cells, endothelial cells and haematopoietic stem cells.

      Clone AV138 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD34 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from Bio-Rad.
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Chicken CD34/huIg fusion protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
      This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Immunocytochemistry(1)
      (1)
      It has been demonstrated that this antibody can be used to detect chicken CD34 expressed in COS7 cells using an AEC substrate detection system.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Clone AV138 detects a band of approximately 70 - 80kDa on COS cell supernatant transfected with chicken CD34 (extracellular)/V5HIS6 tagged protein under reducing conditions.
    • Instructions For Use

    Additional CD34 Antibody Formats

    Formats Clone Applications Sizes available
    CD34 Antibody : Purified AV138 E, IC*, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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