Explore CD68 Antibody Images by Application
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Overview
Bio-Rad provides an extensive collection of antibodies for CD68, with performance guarantee in key immunological applications including Flow Cytometry, Immunohistochemistry, Western Blotting and Immunofluorescence. Listed below is a selection of images, showing the use of CD68 in various applications. The images have been organized by application per species.
A flow cytometry protocol is available that provides detailed procedures for the treatment and staining of this intracellular marker.
Bio-Rad also provides dedicated application pages that have been specifically designed to aid the quick and easy selection of antibodies, kits or reagents per application. These application pages also provide:
- Comprehensive application guides– for in-depth knowledge about a certain application or as a teaching resource
- Detailed information on how to best control& your experiments - to help you obtain publication quality data
- Easy-to-follow protocols– useful when you are performing an application for the first time
- Hands-on tips and tricks to help you improve and optimize your experiments - useful information for both experts and novices
Anti-Mouse CD68
Fig.1. Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with CD68 (MCA1957A488) at a 1/5 dilution and CD88 (MCA2456A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock (BUF041B). Permeabilization was with LeucopermTM (BUF09)
Fig.2. Frozen mouse lymph node stained with Rat Anti-Mouse CD68 (MCA1957) at a 1/100 dilution followed by Goat Anti-Rat IgG:HRP (STAR72) at a 1/50 dilution.
Fig.3. Frozen mouse lymph node stained with Rat Anti-Mouse CD68 (MCA1957) at a 1/100 dilution followed by Goat Anti-Rat IgG:HRP (STAR72) at a 1/50 dilution.
Anti-Rat CD68
Fig.4. Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD11b (MCA275A647) at neat. Isotype control pair in red. Permeabilization was with Leucoperm (BUF09).
Fig.5. Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD163 (MCA342A647) at a 1/5 dilution. Two distinct populations of CD68 positive macrophages were observed, CD163-low M1 macrophages and CD163-high M2 macrophages. Isotype control pair in red. Permeabilization was with Leucoperm (BUF09).
Anti-Human CD68
Fig.6. Dot Blot showing FSC/SSC gated human peripheral blood monocytes dual stained with CD68 (MCA5709F) at neat and CD11b (MCA551A647) at neat. Isotype control pair in red. Permeabilization was with Leucoperm (BUF09).
Fig.7. A, Pacific Blue conjugated Mouse Anti-Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b Isotype Control (MCA691PE). B, Pacific Blue conjugated Mouse Anti-Human CD14 (MCA1568PB) and RPE conjugated Mouse Anti-Human CD68 (MCA6014PE). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.