CD68 antibody | anti-cd68 antibodies

Explore CD68 Antibody Images
by Application

Bio-Rad provides an extensive collection of antibodies for CD68, with performance guarantee in key immunological applications including Flow Cytometry, Immunohistochemistry, Western Blotting and Immunofluorescence. Listed below is a selection of images, showing the use of CD68 in various applications. The images have been organized by application per species.

A flow cytometry protocol is available that provides detailed procedures for the treatment and staining of this intracellular marker.

Bio-Rad also provides dedicated application pages that have been specifically designed to aid the quick and easy selection of antibodies, kits or reagents per application. These application pages also provide:

  • Comprehensive application guides – for in-depth knowledge about a certain application or as a teaching resource
  • Detailed information on how to best control your experiments - to help you obtain publication quality data
  • Easy-to-follow protocols – useful when you are performing an application for the first time
  • Hands-on tips and tricks to help you improve and optimize your experiments - useful information for both experts and novices

Anti-Mouse CD68

J774 cells stained with anti Mouse F4/80 antibody at 1/50 dilution

Fig.1. Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with CD68 (MCA1957A488) at a 1/5 dilution and CD88 (MCA2456A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock (BUF041B). Permeabilization was with LeucopermTM (BUF09)

J774 cells stained with rat anti Mouse F4/80 at 1/10 dilution

Fig.2. Frozen mouse lymph node stained with Rat Anti-Mouse CD68 (MCA1957) at a 1/100 dilution followed by Goat Anti-Rat IgG:HRP (STAR72) at a 1/50 dilution.

J774 cells stained with rat anti Mouse F4/80 at 1/10 dilution

Fig.3. Frozen mouse lymph node stained with Rat Anti-Mouse CD68 (MCA1957) at a 1/100 dilution followed by Goat Anti-Rat IgG:HRP (STAR72) at a 1/50 dilution.


Anti-Rat CD68

Mouse peritoneal macrophages dual stained with F4/80 antibody Alexa Fluor® 647

Fig.4. Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD11b (MCA275A647) at neat. Isotype control pair in red. Permeabilization was with Leucoperm (BUF09).

Mouse peritoneal macrophages dual stained with F4/80 antibody FITC

Fig.5. Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD163 (MCA342A647) at a 1/5 dilution. Two distinct populations of CD68 positive macrophages were observed, CD163-low M1 macrophages and CD163-high M2 macrophages. Isotype control pair in red. Permeabilization was with Leucoperm (BUF09).


Anti-Human CD68

Mouse peritoneal macrophages dual stained with F4/80 antibody APC

Fig.6. Dot Blot showing FSC/SSC gated human peripheral blood monocytes dual stained with CD68 (MCA5709F) at neat and CD11b (MCA551A647) at neat. Isotype control pair in red. Permeabilization was with Leucoperm   (BUF09).

A, Pacific Blue conjugated Mouse Anti-Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b Isotype Control (MCA691PE). B, Pacific Blue conjugated Mouse Anti-Human CD14 (MCA1568PB) and RPE conjugated Mouse Anti-Human CD68 (MCA6014PE). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Fig.7A, Pacific Blue conjugated Mouse Anti-Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b Isotype Control (MCA691PE). B, Pacific Blue conjugated Mouse Anti-Human CD14 (MCA1568PB) and RPE conjugated Mouse Anti-Human CD68 (MCA6014PE). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.



Other related macrophage markers by species reactivity

In addition to CD68, Bio-Rad also offers a wide range of other macrophage markers such as:

Anti-Mouse Antibodies

Anti-Mouse Antibodies

Anti-Human Antibodies

Anti-Human Antibodies

Anti-Rat Antibodies

Anti-Rat Antibodies

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