IgG Antibody

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IgG Antibody gallery image 1

Detection of Rat anti Mouse F4/80 (MCA497 in an immunohistochemical analysis of mouse spleen cryostat sections in the absence [A] or presence [B] of an anti idiotypic anti F4/80 antibody (HCA154) using Goat anti Rat IgG:HRP (STAR72)

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Detection of Rat anti Mouse F4/80 (MCA497GA) in an immunohistochemical analysis of mouse spleen cryostat sections using Goat anti Rat IgG:HRP (STAR72)

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IgG Antibody gallery image 3

Detection of Rat anti Mouse CD169 (MCA884) in an immunohistochemical analysis of a mouse spleen cryosection using Goat anti Rat IgG:HRP (STAR72). High power

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Detection of Rat anti Mouse CD169 (MCA884) in an immunohistochemical analysis of a mouse spleen cryosection using Goat anti Rat IgG:HRP (STAR72). Low power

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Western Blot analysis of CD68 expression on J774 cells using Rat anti mouse CD68 (MCA1957) with Goat anti Rat IgG:HRP (STAR72) as a detection antibody

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Published customer image:
Lipid and macrophage accumulation in lesions of the aortic root. Sequential sections of the aortic root (situated between level 0 and 1) stained for lipid with Sudan IV (A, C, E, and G) or for CD68-positive macrophages (B, D, F, and H) from GLO1TGApoe-/- mice (C, D, G, and H) or nontransgenic Apoe-/- litter mates (A, B, E, and F) that had been administered streptozotocin (E–H) or vehicle (A–D).

From: Geoffrion, M. et al. Differential effects of glyoxalase 1 overexpression on diabetic atherosclerosis and renal dysfunction in streptozotocin-treated, apolipoprotein E-deficient mice. Physiological Reports 2014 Vol. 2 no. e12043.

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Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8α antibody, clone KT15 (MCA609) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) as a detection reagent. Medium power

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Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8α antibody, clone KT15 (MCA609) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) as a detection reagent. High power

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IgG Antibody gallery image 9

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 (a href='https://www.bio-rad-antibodies.com/rat-cd11b-antibody-ed7-mca618r.html'target="_blank">MCA618) followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Medium power

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IgG Antibody gallery image 10

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED7 (MCA618) followed by horseradish peroxidsase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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  • Goat anti Rat IgG:HRP (Mouse Adsorbed)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR72C, E, Pdatasheet pdfdatasheet pdf0.5 mg
    STAR72
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Rat IgG antibody recognizes rat IgG. Cross-reactivity with mouse immunoglobulins has been removed by solid phase adsorption.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Rat IgG
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1000
      Immunohistology - Frozen1/50
      Immunohistology - Paraffin

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP C, E, P 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen

              References

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