IgG antibody

Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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FITC conjugated Goat (F(ab′)2 anti Rat IgG antibody recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues.

Target Species
Rat
Product Form
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Rat IgG.
Approx. Protein Concentrations
F(ab')2 concentration 0.7 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG antibody

  1. Fowles, L. et al. (2000) Regulation of urokinase plasminogen activator gene transcription in the RAW264 murine macrophage cell line by macrophage colony-stimulating factor (CSF-1) is dependent upon the level of cell surface receptor.
    Biochem. J. 347: 313 - 320.
  2. Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
    Br J Cancer. 106: 883-888
  3. Haraoka, M. et al. (1999) Neutrophil recruitment and resistance to urinary tract infection.
    J Infect Dis. 180: 1220-9.
  4. Hang, L. et al. (2000) Interleukin-8 receptor knockout mice have subepithelial neutrophil entrapment and renal scarring following acute pyelonephritis.
    J Infect Dis. 182: 1738-48.
  5. Franke, H. et al. (2005) P2X(7) receptor-mRNA and -protein in the mouse retina; changes during retinal degeneration in BALBCrds mice.
    Neurochem Int. 47: 235-42.
  6. Parish, N.M. et al. (1999) Anti-CD44 treatment does not prevent the extravasation of autopathogenic T cells to the thyroid in experimental autoimmune thyroiditis.
    Immunology. 97: 533-9.
  7. Song, L. et al. (2011) Deletion of the murine scavenger receptor CD68.
    J Lipid Res. 52: 1542-50.
  8. Chapoval, S.P. et al. (2009) Lung vascular endothelial growth factor expression induces local myeloid dendritic cell activation.
    Clin Immunol. 132: 371-84.
  9. View The Latest Product References
  10. Englezou, P.C. et al. (2015) P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages.
    Cytokine. 74 (2): 293-304.

Flow Cytometry

RRID
AB_321934
UniProt
P20759
P20761
P20762
P20760
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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