CD8 Alpha Antibody | YTS105.18

//www.bio-rad-antibodies.com/mouse-cd8-alpha-antibody-yts105-18-mca1108g.html
CD8 Alpha Antibody | YTS105.18 gallery image 1

Staining of mouse spleen cells with Rat anti Mouse CD8 (MCA1108G)

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 2

Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody (MCA74), green in A and Rat anti Mouse CD8 antibody (MCA1108), red in B, Merged image in C

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 3

Published customer image:
Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 µg/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.

From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009) Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4(12): e8291.

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 4

Published customer image:
NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8–positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 µm.

From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 5

Published customer image:
The effect of NK cell or CD8+T cell depletion on NKG2D-positive lymphocyte localization in tumors. Groups of mice (n = 3 per treatment group) were subjected to one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12. Tumor sections were stained as detailed in the Figure 3 legend. (A) Detection of NKG2D-positive cells in tumors from NK cell–depleted mice, (B) Absence of NKG2D-positive cells in CD8+T cell–depleted mice. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 µm.

From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 6

Published customer image:
The effect of NKG2D-blocking antibody on NKG2D-positive CD8+T cells and CD8+T cell accumulation in tumors. 4T-1 tumor–bearing mice were subjected to one of two treatments: Dox plus IL-12 plus control IgG or Dox plus IL-12 plus NKG2D-blocking antibody (n = 3 per treatment). (A) NKG2D expression in CD8+T cells was determined as described for Figure 1 (n.s., not significant). (B) The presence of NKG2D/CD8–positive cells in tumor sections after the indicated treatments were determined as described for Figure 3.

From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 7

Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8α antibody (MCA1108) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) for detection. Low power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 8

Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8α antibody (MCA1108) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) for detection. Medium power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 9

Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8α antibody (MCA1108) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) for detection. High power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 10

Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MCA711), green in A and Rat anti Mouse CD8, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 11

Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MCA711), green in A and Rat anti Mouse CD8, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 12

immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA711), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 13

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MCA1439), green in A and Rat anti Mouse CD8 (MCA1108), red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power

Enlarge
CD8 Alpha Antibody | YTS105.18 gallery image 14

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MCA1439), green in A and Rat anti Mouse CD8 (MCA1108), red in B. Merged image in C wih nuclei counterstained blue using DAPI. High power

Enlarge
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 1
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 2
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 3
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 4
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 5
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 6
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 7
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 8
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 9
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 10
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 11
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 12
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 13
  • CD8 Alpha Antibody | YTS105.18 thumbnail image 14
  • Rat anti Mouse CD8
  • Rat anti Mouse CD8
  • Rat anti Mouse CD8:Low Endotoxin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YTS105.18
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1108GC, F, IFdatasheet pdfdatasheet pdf0.25 mg
    MCA1108G
    MCA1108ELC, F, IFdatasheet pdfdatasheet pdf0.5 mg
    MCA1108EL
    MCA1108GTC, F, IFdatasheet pdfdatasheet pdf25 µg
    MCA1108GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD8, clone YTS105.18 recognizes a non polymorphic epitope on the mouse CD8 alpha chain. This antibody has been reported to block MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies (Cobbold et al. 1990 & Wise et al. 1998).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      None present
    • Immunogen
      Mouse spleen cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cytotoxic T cell differentiation
      positive regulation of calcium-mediated signaling
      cell surface receptor linked signaling pathway
      protein homodimerization activity
      external side of plasma membrane
      integral to membrane
      T cell mediated immunity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Alpha Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Alpha Antibody : Purified YTS105.18 C, F, IF 0.25 mg | 25 µg
    CD8 Alpha Antibody : Low Endotoxin YTS105.18 C, F, IF 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:Low EndotoxinMCA1212EL0.5 mgF
        MCA1212EL

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Write your review

              You may also be interested in...