Rabbit F(ab')2 anti Rat IgG:FITC
- Product Type
- Polyclonal Antibody
- Polyclonal IgG
|Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat IgG.|
- Target Species
- Product Form
- F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer I (FITC) - liquid
- Antiserum Preparation
- Antisera to rat IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
Willheim, M. et al. (1999) Regulatory effects of 1alpha,25-dihydroxyvitamin D3 on the cytokine production of human peripheral blood lymphocytes.
J Clin Endocrinol Metab. 84 (10): 3739-44.
O-Charoenrat, P. et al. (2000) Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
Cancer Res. 60: 1121 - 1128.
Ferreira-Dias G et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
Domest Anim Endocrinol. 29 (1): 203-13.
Thien, R. et al. (2005) Interactions of 1 alpha,25-dihydroxyvitamin D3 with IL-12 and IL-4 on cytokine expression of human T lymphocytes.
J Allergy Clin Immunol. 116 (3): 683-9.
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71.
Agrícola R et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
Reprod Domest Anim. 43 (2): 212-7.
Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
Vet Immunol Immunopathol. 135 (3-4): 275-81.
Leposavić, G. et al. (2010) Age-associated plasticity of α1-adrenoceptor-mediated tuning of T-cell development.
Exp Gerontol. 45 (12): 918-35.
View The Latest Product References
Tiwari, S. (2011) Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.
J Cell Sci. 124: 1672-80.
Van de Velde, H. et al. (2012) Short-term increase of body weight triggers immunological variables in dogs.
Vet Immunol Immunopathol. 145 (1-2): 431-7.
Reynolds, J. et al. (2012) Stimulation of the PD-1/PDL-1 T-cell co-inhibitory pathway is effective in treatment of experimental autoimmune glomerulonephritis.
Nephrol Dial Transplant. 27 (4): 1343-50.
Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
J of Equine Vet Sci. 33 (11): 969-976.
Cian, F. et al. (2014) Stability of immunophenotypic lymphoid markers in fixed canine peripheral blood for flow cytometric analysis.
Vet Clin Pathol. 43 (1): 101-8.
Liu, W. et al. (2015) Evaluation of Stem Cell Marker Expression in Canine B-Cell Lymphoma Cell Lines, B-Cell Lymphoma-generated Spheres and Primary Samples.
Anticancer Res. 35 (5): 2805-16.
Liu, W. et al. (2020) Establishment and characterization of stable red, far-red (fR) and near infra-red (NIR) transfected canine prostate cancer cell lines.
Cancer Cell Int. 20: 139.
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