Antibodies for Kinases

14-3-3 Proteins

Key mediators of Cell Signaling pathways

14-3-3 proteins are of key research interest as they regulate many cellular processes and are highly conserved. There are seven mammalian isoforms of 14-3-3; beta, gamma, zeta, epsilon, tau, eta, and sigma, while the alpha and delta isoforms are phosphorylated.

Bio-Rad’s anti-14-3-3 antibodies are part of our wide Cell Signaling antibody range.

Our 14-3-3 Antibody Range

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    Western blot

    14-3-3 proteins form dimers that regulate many cellular processes through their interaction with a wide variety of ligands such as

    Research studies suggest that 14-3-3 isoforms are differentially regulated in various diseases, particularly in:

    • Cancer - Expression of 14-3-3 sigma is lost in carcinoma of the breast, ovary, endometrium, prostate, liver, skin and lung.
    • Neurological disorders - 14-3-3 eta forms part of Lewy bodies in Parkinson’s disease and is also linked to early onset schizophrenia. 14-3-3 is also linked to Alzheimer’s disease and prion diseases such as CJD.

    You can complement your 14-3-3 research with reagents from our Oncoprotein and Tumour Suppressor protein, P53 and extensive Neurology antibody ranges.


    References:

    1. Aitken, A. et. al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16:162-172.
    2. Yang, H. et. al. (2006) DNA damage induced Protein 14-3-3 (sigma) inhibits Protein Kinase B/AKT activation and suppresses Akt-activated cancer. Cancer Res. 66:3096-3105.
    3. Dougherty, M.K. et. al. (2004) Unlocking the code of 14-3-3. J. Cell Science 117:1875-1884.
    4. Woodcock, J.M. et. al. (2003) The dimeric versus monomeric status of 14-3-3 zeta is controlled by phosphorylation of Ser58 at the dimer interface. J. Biol. Chem. 278:36323-36327.
    5. Jones, H. et. al. (1995) Expression and Structural Analysis of 14-3-3 Proteins. J. Mol. Biol. 245:375-384.
    6. Martin, H. et. al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein. FEBS 331:296-303.
    7. Boston et. al. (1982) Journal of Neurochemistry 38:1466-1474.