BAD antibody
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WB P IF ResetRabbit anti BAD
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- BAD
Antibody Quality and 
Unrivaled 
| Rabbit anti BAD antibody recognizes human Bcl2-associated agonist of cell death (BAD), also known as Bcl-xL/Bcl-2-associated death promoter, Bcl-2 binding component 6 or Bcl2-L-8. Similar to the other Bcl-2 protein family members, Bax, Bak, Bid and Bock, BAD has a pro-apoptotic function in the apoptosis signaling cascade. The precise role of BAD in apoptosis signaling depends on BAD’s post-translational modification status. Nonphosphorylated BAD forms heterodimers with the two anti-apoptotic Bcl-2 protein family members, Bcl-xL and Bcl-2 (Zha et al. 1996). The binding of BAD to Bcl-xL promotes apoptosis by inhibiting the apoptosis repressor function of Bcl-xL (Yang et al. 1995). Once serine phosphorylated, BAD is unable to heterodimerize with Bcl-xL and interacts with 14-3-3 proteins instead (Masters et al. 1995). This binding retains BAD in the cytoplasm and thereby enables Bcl-xL to suppress apoptosis (Zha et al. 1996). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BAD was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Synthetic human BAD peptide
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
1/10 | 1/100 |
| Immunohistology - Paraffin | |
1/50 | 1/200 |
| Western Blotting | |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti BAD antibody detects a band of approximately 18 kDa in PC3 cell lysate under reducing conditions
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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References for BAD antibody
-
Zha, J. et al. (1996) Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L).
Cell 87 (4): 619-28. -
Yang, E. et al. (1995) Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death.
Cell 80 (2): 285-91. -
Masters, S.C. et al. (2001) 14-3-3 inhibits Bad-induced cell death through interaction with serine-136.
Mol. Pharmacol. 60 (6): 1325-31.
- UniProt
- Q92934
- Entrez Gene
- BAD
- GO Terms
- GO:0005829 cytosol
- GO:0005741 mitochondrial outer membrane
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0019901 protein kinase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0048011 nerve growth factor receptor signaling pathway
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