Thyroxine Antibody | BGN/0980/322 (T4:1-32.2)

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Thyroxine Antibody | BGN/0980/322 (T4:1-32.2) gallery image 1

Published customer image:
Mouse anti Thyroxine antibody, clone BGN/0980/322 use for the detection of thyroxine in mouse thyroid tissue by immunofluorescence.
Image caption:
Thyroid morphology and expression of thyrocyte and C-cell markers at late embryonic stages. Staining for Nkx2-1 (A, B, F, G, K, L, P, Q), Pax8 and TG (C, H, M, R), CT and T4 (D, I, N, S), and Mash1 (E, J, O, T) at E16.5 (A–J) and E15.5 (K–T) in transverse sections from wild type and Hes1-/- mice. Boxed areas were enlarged (B–E, G–J, L–O, Q–T). Thyroid fusion was delayed by 3 days in Hes1-/- thyroids compared to wild type thyroids. tr: trachea; thy: thyroid; ma: median anlage; pt: parathyroid; UB: ultimobranchial body.

From: Carre A, Rachdi L, Tron E, Richard B, Castanet M, et al. (2011) Hes1 Is Required for Appropriate Morphogenesis and Differentiation during Mouse Thyroid Gland Development. PLoS ONE 6(2): e16752.

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  • Mouse anti Thyroxine
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  • Product Type
    Monoclonal Antibody
  • Clone
    BGN/0980/322 (T4:1-32.2)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    8959-9831E, IFdatasheet pdfdatasheet pdf1 mg
    8959-9831
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 8959-9831 is specific for thyroxine, a form of thyroid hormone secreted by the follicular cells of the thyroid gland. 8959-9831 does not cross react with either rT3 or T3.
    • Intended Use
    • Target Species
      Broad
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      flounder (Paralichthys olivaceus)yes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Thyroxine conjugated to Bovine Serum Albumin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/201/200
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Thyroxine Antibody Formats

    Formats Clone Applications Sizes available
    Thyroxine Antibody : Purified BGN/0980/322 (T4:1-32.2) E, IF 1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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