Strep-Tag Immo Antibody | Strep-MAB

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  • Mouse anti Strep-Tag Immo
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Strep-MAB
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2488Edatasheet pdfdatasheet pdf0.1 mg
    MCA2488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Clone Strep-MAB, also known as StrepMAB-Immo, recognizes Strep-tag II sequence WSHPQFEK, a widely used tag in protein expression applications.

      This antibody demonstrates near irreversible binding of Strep-tag II, which is N-terminally extended by SerAla. This makes the antibody particularly suited to use in assays such as ELISA and BiaCore.

      This product is not recommended for the detection of Strep II fusion proteins in Western blot.
    • Intended Use
    • Target Species
      Synthetic Peptide
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml PBS.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein SA-WSHPQFEK
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Ammonium bicarbonate
      1% Sucrose
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of reconstitution.
    • Acknowledgements
      Sold under license of IBA
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Strep-Tag Immo Antibody Formats

    Formats Clone Applications Sizes available
    Strep-Tag Immo Antibody : Purified Strep-MAB E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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