CD230 Antibody | ROS-BC6

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CD230 Antibody | ROS-BC6 gallery image 1

Western Blot analysis of various tissues using Mouse anti Sheep CD230, clone ROS-BC6 (MCA6009). Lane 1 PRPc BSE infected ovine medulla, Lane 2 PRPc unifected ovine medulla, Lane 3 Scrapie infected ovine medulla, Lane 4 PRPsc scrapie infected ovine spleen, Lane 5 PRPsc BSE infected ovine medulla, Lane 6 PRPsc BSE infected ovine spleen. Image courtesy of The Roslin Institute

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CD230 Antibody | ROS-BC6 gallery image 2

Flow cytometry analysis of CD230. Ovine PBMC's staining using Mouse anti Sheep CD230, clone ROS-BC6 (MCA6009) as the primary antibody followed by an anti mouse IgG conjugated to APC. Gated on sytox blue negative live cells and the PBMC region determined by FSC v. SSC profile. Image courtesy of The Roslin Institute.

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CD230 Antibody | ROS-BC6 gallery image 3

Flow cytometry analysis of ovine PBMC's using Mouse anti Sheep CD230, clone ROS-BC6 (MCA6009). Images courtesy of the Roslin Institute

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  • CD230 Antibody | ROS-BC6 thumbnail image 1
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  • Mouse anti Sheep CD230 (aa144 - aa154)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ROS-BC6
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6009GAF, P, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA6009GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Sheep CD230 (Prpsc) antibody, clone ROS-BC6 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals and known as Scrapie (sheep and goats), BSE (cattle), CWD (cervine/deer ) and CJD (humans). These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioral changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.

      CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Humanno
      Hamsteryes
      Mouseyes
      Red deeryes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      A truncated form of recombinant Ovine PRP spanning residues aa94 - aa233.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised 129/Ola mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      anchored to membrane
      Golgi apparatus
      copper ion binding
      plasma membrane
      protein homooligomerization
      microtubule binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This reagent was licenced from The Roslin Institute at the University of Edinburgh as part of the immunological toolbox project
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD230 Antibody Formats

    Formats Clone Applications Sizes available
    CD230 Antibody : Purified ROS-BC6 F, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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