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TCR Gamma/Delta antibody | V65

Mouse anti Rat TCR Gamma/Delta

Product Type
Monoclonal Antibody
Clone
V65
Isotype
IgG1
Specificity
TCR Gamma/Delta

Product Code Applications Pack Size List Price Your Price Qty
MCA1146
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
List Price Your Price
loader

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 recognizes a constant determinant of the rat gamma/delta T cell receptor.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Prosep A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
CD3 +ve TCR alpha/beta -ve rat T cell hybridoma line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag:8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

Source Reference

  1. Kühnlein, P. et al. (1994) Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
    J Immunol. 153 (3): 979-86.

References for TCR Gamma/Delta antibody

  1. Kühnlein, P. et al. (1995) Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs.
    Dev Immunol. 4 (3): 181-8.
  2. Yrlid, U. et al. (2006) Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.
    J Immunol. 177: 6115-21.
  3. Martin, A. et al. (2018) Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment.
    Oncotarget. 9 (47): 28364-78.

RRID
AB_322446

MCA1146

149734 166402

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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