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CD47 antibody | OX-101

Product Code Applications Pack Size List Price Your Price Qty
MCA1997
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB 0.25 mg loader
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loader

Mouse anti Rat CD47 antibody, clone OX-101 recognizes the rat homologue of human CD47, also known as integrin associated protein (IAP). Rat CD47 is a 303 amino acid ӯ33 kDa multi pass transmembrane glycoprotein bearing a single V-type Ig-like domain and has been identified as the ligand for the rat signal regulatory protein, SIRP (Vernon-Wilson et al. 2000). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.

Mouse anti Rat CD47 antibody, clone OX-101 has been reported to block the interaction of CD47 and SIRP (Vernon-Wilson et al. 2000).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Rat thymocytes.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Mouse IgG1 Negative Control

References for CD47 antibody

  1. Vernon-Wilson, E.F. et al. (2000) CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1.
    Eur J Immunol. 30 (8): 2130-7.
  2. de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
    J Immunol. 168 (11): 5832-9.
  3. Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells.
    Cell Mol Life Sci. 66: 1271-82.
  4. Barrès, C. et al. (2010) Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages.
    Blood. 115: 696-705.
  5. Kurihara H et al. (2010) SIRP-alpha-CD47 system functions as an intercellular signal in the renal glomerulus.
    Am J Physiol Renal Physiol. 299 (3): F517-27.

Synonyms
OX41 Ligand
SIRP LIGAND
RRID
AB_2074952
UniProt
P97829
Entrez Gene
Cd47
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007229 integrin-mediated signaling pathway
GO:0016477 cell migration

MCA1997

154551 1707

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