CD45RC Antibody | OX-22

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CD45RC Antibody | OX-22 gallery image 1

Staining of rat spleen cells with MOUSE.ANTI RAT CD45RC:Alexa Fluor® 647 (MCA53A647)

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CD45RC Antibody | OX-22 gallery image 2

Staining of rat spleen cells with MOUSE.ANTI RAT CD45RC:FITC (MCA53FT)

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  • CD45RC Antibody | OX-22 thumbnail image 1
  • CD45RC Antibody | OX-22 thumbnail image 2
  • Mouse anti Rat CD45RC:Low Endotoxin
  • Mouse anti Rat CD45RC:Alexa Fluor® 647
  • Mouse anti Rat CD45RC
  • Mouse anti Rat CD45RC
  • Mouse anti Rat CD45RC:FITC
  • Mouse anti Rat CD45RC
  • Mouse anti Rat CD45RC:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-22
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA53GAC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA53GA
    MCA53FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA53FT
    MCA53RC, F, P *datasheet pdfdatasheet pdf0.25 mg
    MCA53R
    MCA53ELC, F, P *datasheet pdfdatasheet pdf0.5 mg
    MCA53EL
    MCA53GC, F, P *datasheet pdfdatasheet pdf1 mg
    MCA53G
    MCA53PEFdatasheet pdfdatasheet pdf100 Tests
    MCA53PE
    MCA53A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA53A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD45RC antibody, clone OX-22 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).

      This product is routinely tested in flow cytometry on rat splenocytes.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G
      Pack Size: 1 mg, 0.25 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 1 mg, 0.25 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G
      Pack Size: 1 mg, 0.25 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      PHA stimulated rat lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 1 mg, 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      Pack Size: 1 mg, 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      Pack Size: 1 mg, 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      release of sequestered calcium ion into cytosol
      B cell receptor signaling pathway
      focal adhesion
      transmembrane receptor protein tyrosine phosphatase activity
      positive regulation of antigen receptor-mediated signaling pathway
      defense response to virus
      negative regulation of T cell mediated cytotoxicity
      T cell receptor signaling pathway
      B cell proliferation
      protein dephosphorylation
      protein kinase binding
      response to gamma radiation
      integral to plasma membrane
      negative regulation of protein kinase activity
      negative regulation of cytokine-mediated signaling pathway
      internal side of plasma membrane
      membrane raft
      protein kinase regulator activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen1/1001/1000
      Immunohistology - Paraffin(1)1/1001/1000
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen1/1001/1000
      Immunohistology - Paraffin(1)1/1001/1000
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNEAT1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen1/1001/1000
      Immunohistology - Paraffin(1)1/1001/1000
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45RC Antibody Formats

    Formats Clone Applications Sizes available
    CD45RC Antibody : RPE OX-22 F 100 Tests
    CD45RC Antibody : Purified OX-22 C, F, P * 1 mg | 0.1 mg | 0.25 mg
    CD45RC Antibody : Low Endotoxin OX-22 C, F, P * 0.5 mg
    CD45RC Antibody : Alexa Fluor® 647 OX-22 F 100 Tests/1ml
    CD45RC Antibody : FITC OX-22 F 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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